| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,937,083 | A→T | T330T (ACA→ACT) | fucK → | L‑fuculokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,937,083 | 0 | A | T | 86.7% | 37.3 / ‑0.2 | 15 | T330T (ACA→ACT) | fucK | L‑fuculokinase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (13/0); total (14/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAA > W3110S.gb/2937031‑2937142 | tggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACTCCCTGGCa > 1:1161435/1‑61 (MQ=255)tggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACTCCCTGGCa > 1:935133/1‑61 (MQ=255)tggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACTCCCTGGCa > 1:3104926/1‑61 (MQ=255)tggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACTCCCTGGCa > 1:3037975/1‑61 (MQ=255)tggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACTCCCTGGCa > 1:295981/1‑61 (MQ=255)tggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACTCCCTGGCa > 1:2713969/1‑61 (MQ=255)tggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACTCCCTGGCa > 1:2705168/1‑61 (MQ=255)tggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACTCCCTGGCa > 1:2603811/1‑61 (MQ=255)tggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACTCCCTGGCa > 1:2565023/1‑61 (MQ=255)tggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACTCCCTGGCa > 1:2284854/1‑61 (MQ=255)tggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACTCCCTGGCa > 1:2132341/1‑61 (MQ=255)tggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACTCCCTGGCa > 1:1705518/1‑61 (MQ=255)tggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACTCCCTGGCa > 1:1668727/1‑61 (MQ=255) aaaaCTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATcg < 1:1728676/62‑1 (MQ=255) aaCTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGt > 1:3019978/1‑53 (MQ=255) acacCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTaaa < 1:1008370/62‑1 (MQ=255) acacCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTa < 1:498830/60‑1 (MQ=255) acacCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTa < 1:947514/60‑1 (MQ=255) | TGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAA > W3110S.gb/2937031‑2937142 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |