| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,939,036 | C→T | L288L (TTG→TTA) | ygdE ← | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,939,036 | 0 | C | T | 78.3% | 39.5 / 8.4 | 24 | L288L (TTG→TTA) | ygdE | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (3/2); new base T (18/0); total (21/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
GGTTGAAAATGGTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATACCATCC > W3110S.gb/2938987‑2939082 | ggTTGAAAATGGTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATGAATGCCGCAAc < 1:1169352/61‑1 (MQ=255)ggTTGAAAATGGTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAAc < 1:1175138/61‑1 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:2788454/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:815689/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:3093490/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:3089270/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:2898103/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:2877545/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:2872394/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:279141/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:2784979/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:2691763/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:2322809/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:2095731/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:2068080/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:2058321/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:1678192/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:1585632/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATTAATGCCGCAACTTTAGCCGGtt > 1:60438/2‑62 (MQ=255) agTTTCACGGCACCAGCCATTAACCAGACACTGCGCCATTAATGCCGCAACTTTCGCCGGtt > 1:3011849/2‑62 (MQ=255) ttAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATAc < 1:975256/62‑1 (MQ=255) cACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATACCATcc > 1:2843607/1‑58 (MQ=255) cACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATACCATcc > 1:1753/1‑58 (MQ=255) cACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATACCATcc > 1:1558989/1‑58 (MQ=255) | GGTTGAAAATGGTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATACCATCC > W3110S.gb/2938987‑2939082 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |