Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,089,035	A→G	V11V (GTA→GTG)	endA →	DNA‑specific endonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,089,035	0	A	G	100.0%	56.3 / NA	17	V11V (GTA→GTG)	endA	DNA‑specific endonuclease I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/9);  total (8/9)

CGTTATTTGTCTATTGCTGCGGTGGTACTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGTATCAATAGTTTTT  >  W3110S.gb/3089009‑3089087                           |                                                     cGTTATTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGa                   >  1:2235375/1‑62 (MQ=255) cGTTATTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGa                   >  1:2640424/1‑62 (MQ=255) cGTTATTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGa                   >  1:2741555/1‑62 (MQ=255) cGTTATTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGa                   >  1:411295/1‑62 (MQ=255)   ttATTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAg                 <  1:1078413/62‑1 (MQ=255)   ttATTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAg                 <  1:1853248/62‑1 (MQ=255)   ttATTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAg                 <  1:1956891/62‑1 (MQ=255)   ttATTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAg                 <  1:2147844/62‑1 (MQ=255)   ttATTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAg                 <  1:2343752/62‑1 (MQ=255)   ttATTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAg                 <  1:2697961/62‑1 (MQ=255)   ttATTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAg                 <  1:2787237/62‑1 (MQ=255)   ttATTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAg                 <  1:2893519/62‑1 (MQ=255)   ttATTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAg                 <  1:517940/62‑1 (MQ=255)     aTTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGt               >  1:1534460/1‑62 (MQ=255)     aTTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGt               >  1:852289/1‑62 (MQ=255)     aTTTGTCTATTGCTGCGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGt               >  1:2523722/1‑62 (MQ=255)                   gcGGTGGTGCTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGTATCAATAGttttt  >  1:902279/1‑61 (MQ=255)                           |                                                     CGTTATTTGTCTATTGCTGCGGTGGTACTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGTATCAATAGTTTTT  >  W3110S.gb/3089009‑3089087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑