Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	68,955	2 bp→TC	coding (1093‑1094/1701 nt)	araB ←	L‑ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	68,955	0	G	T	100.0%	75.7 / NA	25	T365E (ACG→GAG)	araB	L‑ribulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/25);  total (0/25)
*	W3110S.gb	68,956	0	T	C	100.0%	84.6 / NA	25	T365E (ACG→GAG)	araB	L‑ribulokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/25);  total (0/25)

CAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTC  >  W3110S.gb/68915‑68960                                         ||     cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:2648612/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:967339/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:829616/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:683996/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:623642/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:510329/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:3081527/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:2962828/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:2938400/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:2905848/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:2905296/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:2697704/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:1070503/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:2584562/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:253626/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:2504204/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:2432792/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:2428010/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:231274/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:2140605/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:1799976/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:179587/46‑1 (MQ=38) cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:1611493/46‑1 (MQ=38)  aGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:1997321/45‑1 (MQ=38)  aGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCTCTTTc  <  1:736593/45‑1 (MQ=38)                                         ||     CAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTC  >  W3110S.gb/68915‑68960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑