| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,659,831 | 2 bp→AA | coding (572‑573/1251 nt) | wzxE ← | O‑antigen translocase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,659,831 | 0 | G | A | 95.5% | 133.8 / ‑1.7 | 44 | A191V (GCC→GTT) | wzxE | O‑antigen translocase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (42/0); total (43/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
| * | W3110S.gb | 3,659,832 | 0 | G | A | 84.0% | 115.3 / 15.0 | 50 | A191V (GCC→GTT) | wzxE | O‑antigen translocase |
| Reads supporting (aligned to +/- strand): ref base G (7/1); new base A (42/0); total (49/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
TATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCATAACC > W3110S.gb/3659773‑3659893 || tATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATggc > 1:435429/1‑61 (MQ=255)tATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATggc > 1:2643187/1‑61 (MQ=255) gggATGACACCACGTTTGATCAACATGATGGCGGCAGGa > 1:1749227/1‑39 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAGTTACCACCAGCGCGGGAATCAgcgc > 1:2182333/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:2917134/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:2026686/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:2117113/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:2280043/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:2348655/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:2367994/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:2436253/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:2506348/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:2589145/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:2593645/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1011386/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:354342/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:362496/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:513960/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:733462/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:833855/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:861890/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:901173/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:912172/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:948078/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:981523/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1974630/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1422134/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1335253/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1493599/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1318952/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1267539/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1532401/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1589848/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:166235/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1674158/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1240901/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1697437/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1767432/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1777830/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:178223/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1178782/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1826684/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:183791/1‑60 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGCGCGGGAATCAgcg > 1:449877/1‑59 (MQ=255) gACACCACGTTTGATCAACATGATAACAGCAGGAATTACCACCAGAGCGGGAATCAgcg > 1:1864688/1‑59 (MQ=255) cGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCgg < 1:2337825/43‑1 (MQ=255) tgGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGAccc > 1:74753/1‑44 (MQ=255) tgGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGccc > 1:799071/1‑54 (MQ=255) tgGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGccc > 1:2960512/1‑54 (MQ=255) tgGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGccc > 1:2944920/1‑54 (MQ=255) tgGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGccc > 1:2580817/1‑54 (MQ=255) tgGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGccc > 1:1207528/1‑54 (MQ=255) gcggcAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCATAAcc > 1:1355745/1‑62 (MQ=255) gcggcAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCATAAcc > 1:70303/1‑62 (MQ=255) gcggcAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCATAAcc > 1:864696/1‑62 (MQ=255) gcggcAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCATAAcc > 1:1006622/1‑62 (MQ=255) gcggcAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCATCATAAcc > 1:1447934/1‑62 (MQ=255) gcggcAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCAAGCAACGCCCCt > 1:550307/1‑54 (MQ=255) || TATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCATAACC > W3110S.gb/3659773‑3659893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |