Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,870,366	2 bp→GC	coding (1745‑1746/1935 nt)	yeaG →	conserved hypothetical protein with nucleoside triphosphate hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,870,366	0	C	G	91.7%	32.0 / ‑3.4	12	T582S (ACG→AGC)	yeaG	conserved hypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (11/0);  total (11/1)
Fisher's exact test for biased strand distribution p-value = 8.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
*	W3110S.gb	1,870,367	0	G	C	91.7%	32.6 / ‑3.2	12	T582S (ACG→AGC)	yeaG	conserved hypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (11/0);  total (11/1)
Fisher's exact test for biased strand distribution p-value = 8.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

CGCGCCAGAGCGAATAACAGCGGACGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAAC  >  W3110S.gb/1870305‑1870427                                                              ||                                                             cgcgcCAGAGCGAATAACAGCGGACGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCAc                                                               >  1:552941/1‑62 (MQ=255)                            aaTCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCaa                                    <  1:2495472/62‑1 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTtctcga                                     >  1:2782153/1‑50 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa                                    >  1:125380/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa                                    >  1:170733/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa                                    >  1:1978902/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa                                    >  1:2134898/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa                                    >  1:2510989/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa                                    >  1:2763785/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa                                    >  1:2783941/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa                                    >  1:3098013/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa                                    >  1:416443/1‑53 (MQ=255)                                     tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa                                    >  1:612996/1‑53 (MQ=255)                                                               ggTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAAc  >  1:1713915/1‑61 (MQ=255)                                                              ||                                                             CGCGCCAGAGCGAATAACAGCGGACGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAAC  >  W3110S.gb/1870305‑1870427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑