Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,682,030	Δ2 bp	coding (512‑513/1194 nt)	yphH →	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,682,029	0	G	.	100.0%	29.3 / NA	8	coding (511/1194 nt)	yphH	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (8/0);  total (8/0)
*	W3110S.gb	2,682,030	0	A	.	100.0%	29.3 / NA	8	coding (512/1194 nt)	yphH	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (8/0);  total (8/0)

GTGACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTGGAGGTGAAATATC  >  W3110S.gb/2681978‑2682041                                                    ||            gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAatat   >  1:2549305/1‑61 (MQ=255) gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAatat   >  1:2886660/1‑61 (MQ=255) gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAatat   >  1:958036/1‑61 (MQ=255) gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAATATc  >  1:1453928/1‑62 (MQ=255) gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAATATc  >  1:1661006/1‑62 (MQ=255) gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAATATc  >  1:181344/1‑62 (MQ=255) gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAATATc  >  1:2272169/1‑62 (MQ=255) gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAATATc  >  1:509444/1‑62 (MQ=255)                                                    ||            GTGACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTGGAGGTGAAATATC  >  W3110S.gb/2681978‑2682041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑