Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,815,248	(A)8→7	coding (349/429 nt)	yqaA ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,815,241	0	A	.	100.0%	79.1 / NA	19	coding (356/429 nt)	yqaA	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (17/2);  total (17/2)

CGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCACAAAAAAAAGATTACCGGTCCCCACGA  >  W3110S.gb/2815194‑2815266                                                |                          cGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTAc              <  1:1287220/60‑1 (MQ=255) cGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTAc              <  1:512269/60‑1 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:2077809/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:851637/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:829299/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:484913/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:468774/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:349703/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:2827604/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:2213554/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:1257842/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:2059532/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:1988710/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:1762153/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:1590021/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:1468817/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACga  >  1:1263577/1‑61 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACg   >  1:660019/1‑60 (MQ=255)            gcaactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACg   >  1:1270845/1‑60 (MQ=255)                                                |                          CGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCACAAAAAAAAGATTACCGGTCCCCACGA  >  W3110S.gb/2815194‑2815266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑