Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,058,102	Δ1 bp	coding (453/1179 nt)	yhhZ ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,058,100	0	G	.	82.3%	84.9 / 22.6	34	coding (455/1179 nt)	yhhZ	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/6);  new base . (0/28);  total (0/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

TAAAAACAAACGGTTATAATTTTCAGGGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACA  >  W3110S.gb/4058075‑4058146                          |                                               tAAAAACAAACGGTTATAATTTTCAGGGAGTGCCAAATAGCTGAACTCGGTATTTTCGATa             <  1:588817/61‑1 (MQ=255) tAAAAACAAACGGTTATAATTTTCAGGGAGTGCCAAATAGCTGAACTCGGTATTTGCGATa             <  1:1485451/61‑1 (MQ=255) tAAAAACAAACGGTTATAATTTTCAGGGAGTGCCAAATAGCTGAACTCGGTATTTGCGATa             <  1:1556216/61‑1 (MQ=255) tAAAAACAAACGGTTATAATTTTCAGGGAGTGCCAAATAGCTGAACTCGGTATTTGCGATa             <  1:701847/61‑1 (MQ=255) tAAAAACAAACGGTTATAATTTTCAGGGAGTGCCAAATAGCTGAACTCGGTATTTGCGATa             <  1:2736321/61‑1 (MQ=255)   aaaaCAAACGGTTATAATTTTCAGGGAGTGCCAAATAGCTGAACTCGGTATTTGCGATa             <  1:2257004/59‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCTGTATTTGCGATAAGATGTTGACa  <  1:316909/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:408083/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:2651721/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:2695079/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:2833230/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:346706/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:394265/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:1222191/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:461494/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:686365/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:780404/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:794624/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:2329283/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:2215679/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:2070764/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:1849032/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:1843147/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:1799745/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:1573490/62‑1 (MQ=255)          acGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:1351785/62‑1 (MQ=255)           cGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:2343540/61‑1 (MQ=255)           cGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:2789273/61‑1 (MQ=255)           cGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:2001841/61‑1 (MQ=255)           cGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:1948410/61‑1 (MQ=255)           cGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:59318/61‑1 (MQ=255)           cGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:1572354/61‑1 (MQ=255)           cGGTTATAATTTTCA‑GGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:1213775/61‑1 (MQ=255)           cGGTTATAATTTTCA‑GGAGTGCCAAATAGCAGAACTCGGTATTTGCGATAAGATGTTGACa  <  1:256658/61‑1 (MQ=255)                          |                                               TAAAAACAAACGGTTATAATTTTCAGGGAGTGCCAAATAGCTGAACTCGGTATTTGCGATAAGATGTTGACA  >  W3110S.gb/4058075‑4058146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑