Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	932,590	Δ1 bp	intergenic (‑118/‑427)	trxB ← / → lrp	thioredoxin reductase, FAD/NAD(P)‑binding/DNA‑binding transcriptional dual regulator, leucine‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	932,590	0	T	.	100.0%	24.4 / NA	7	intergenic (‑118/‑427)	trxB/lrp	thioredoxin reductase, FAD/NAD(P)‑binding/DNA‑binding transcriptional dual regulator, leucine‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (7/0);  total (7/0)

TGTTAACAATTTGTGCAATCGGCAGCATCGATAAGCAGGTCAAATTCTCCCGTCATTATCACC  >  W3110S.gb/932551‑932613                                        |                        tGTTAACAATTTGTGCAATCGGCGGCATCGATAAGCAGG‑CAAATTCTccc              >  1:1700386/1‑50 (MQ=255) tGTTAACAATTTGTGCAATCGGCAGCATCGATAAGCAGG‑CAAATTCTCCCGTCATTATCAcc  >  1:101175/1‑62 (MQ=255) tGTTAACAATTTGTGCAATCGGCAGCATCGATAAGCAGG‑CAAATTCTCCCGTCATTATCAcc  >  1:1179531/1‑62 (MQ=255) tGTTAACAATTTGTGCAATCGGCAGCATCGATAAGCAGG‑CAAATTCTCCCGTCATTATCAcc  >  1:2027026/1‑62 (MQ=255) tGTTAACAATTTGTGCAATCGGCAGCATCGATAAGCAGG‑CAAATTCTCCCGTCATTATCAcc  >  1:742890/1‑62 (MQ=255) tGTTAACAATTTGTGCAATCGGCAGCATCGATAAGCAGG‑CAAATTCTCCCGTCATTATCAc   >  1:1552944/1‑61 (MQ=255) tGTTAACAATTTGTGCAATCGGCAGCATCGATAAGCAGG‑CAAATTCTCCCGTCATTATCAc   >  1:2045262/1‑61 (MQ=255)                                        |                        TGTTAACAATTTGTGCAATCGGCAGCATCGATAAGCAGGTCAAATTCTCCCGTCATTATCACC  >  W3110S.gb/932551‑932613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑