Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,093,733	+T	coding (436/1359 nt)	ycdT →	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,093,729	1	.	T	90.0%	24.3 / ‑2.3	10	coding (432/1359 nt)	ycdT	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (9/0);  total (10/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTA‑TTTTCCAAAAAAATGACTTTGTGCATAAG  >  W3110S.gb/1093670‑1093758                                                             |                              atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta‑tt                             >  1:1046366/1‑62 (MQ=255) atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta‑tt                             >  1:156911/1‑62 (MQ=255) atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta‑tt                             >  1:3839/1‑62 (MQ=255) atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta‑tt                             >  1:525005/1‑62 (MQ=255)                            aaaGTTAGTGAAAACACAAAACAGCGCAATTTATTTTTCCAAAAAAATGACTTTGTGCATaa   >  1:1130205/1‑62 (MQ=255)                            aaaGTTAGTGAAAACACAAAACAGCGCAATTTATTTTTCCAAAAAAATGACTTTGTGCATaa   >  1:1904542/1‑62 (MQ=255)                            aaaGTTAGTGAAAACACAAAACAGCGCAATTTATTTTTCCAAAAAAATGACTTTGTGCATaa   >  1:2343282/1‑62 (MQ=255)                            aaaGTTAGTGAAAACACAAAACAGCGCAATTTATTTTTCCAAAAAAATGACTTTGTGCATaa   >  1:2769955/1‑62 (MQ=255)                            aaaGTTAGTGAAAACACAAAACAGCGCAATTTATTTTTCCAAAAAAATGACTTTGTGCATaa   >  1:2814756/1‑62 (MQ=255)                            aaaGTTAGTGAAAACACAAAACAGCGCAATTTATTTTTCCAAAAAAATGACTTTGTGCATaa   >  1:374359/1‑62 (MQ=255)                            aaaGTTAGTGAAAACACAAAACAGCGCAATTTATTTTTCCAAAAAAATGACTTTGTGCATaa   >  1:382430/1‑62 (MQ=255)                            aaaGTTAGTGAAAACACAAAACAGCGCAATTTATTTTTCCAAAAAAATGACTTTGTGCATaa   >  1:705930/1‑62 (MQ=255)                            aaaGTTAGTGAAAACACAAAACAGCGCAATTTATTTTTCCAAAAAAATGACTTTGTGCATaa   >  1:942792/1‑62 (MQ=255)                            aaaGTTAGTGAAAACACAAAACAGCGCAATTTA‑TTTTCCAAAAAAATGACTTTGTGCATAAg  >  1:2173806/1‑62 (MQ=255)                                                             |                              ATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTA‑TTTTCCAAAAAAATGACTTTGTGCATAAG  >  W3110S.gb/1093670‑1093758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑