| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,420,329 | +C | intergenic (‑386/‑56) | kil ← / → sieB | inhibitor of ftsZ, killing protein/phage superinfection exclusion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,420,325 | 1 | . | C | 79.2% | 62.2 / 12.2 | 24 | intergenic (‑382/‑60) | kil/sieB | inhibitor of ftsZ, killing protein/phage superinfection exclusion protein |
| Reads supporting (aligned to +/- strand): ref base . (0/5); new base C (0/19); total (0/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTG > W3110S.gb/1420283‑1420372 | ggTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTtg < 1:964335/62‑1 (MQ=255)ggTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTtg < 1:864461/62‑1 (MQ=255)ggTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTtg < 1:1342421/62‑1 (MQ=255)ggTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTtg < 1:1671889/62‑1 (MQ=255) caccTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTtg < 1:1661679/49‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:2061772/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:835574/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:736690/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:645449/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:515139/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:2820956/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:2713658/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:2674540/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:2514259/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:1047443/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:190992/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:1755601/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:1544578/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:15111/62‑1 (MQ=255) tggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:150610/62‑1 (MQ=255) ggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:1908064/61‑1 (MQ=255) ggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:371740/61‑1 (MQ=255) ggtCTCGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:1320862/61‑1 (MQ=255) gggctcGGCTATACCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTg < 1:1309960/58‑1 (MQ=255) | GGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATA‑CCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTG > W3110S.gb/1420283‑1420372 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |