Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,224,964	+G	coding (360/2298 nt)	bglX ←	beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,224,961	1	.	G	100.0%	77.1 / NA	24	P121P (CCG→CCC)	bglX	beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (0/24);  total (0/24)

GAGGCTAATC‑GGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAA  >  W3110S.gb/2224952‑2225013           |                                                     gAGGCTAATCGGGGAACACCGTGCGCT‑ACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:1445079/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGTaa  <  1:316329/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:1932543/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:723803/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:65814/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:416160/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:358572/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:2653461/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:2539446/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:2471892/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:2319225/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:2195629/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:2188910/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:1170871/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:1863102/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:179262/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:171204/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:1649891/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:1569290/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:155185/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:1357973/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:1338249/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:1283739/62‑1 (MQ=255)  aGGCTAATCGGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGaa  <  1:1224511/62‑1 (MQ=255)           |                                                     GAGGCTAATC‑GGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAA  >  W3110S.gb/2224952‑2225013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑