Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 2,837,372 | +A | coding (552/1014 nt) | ascG ← | DNA‑binding transcriptional regulator |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 2,837,369 | 1 | . | A | 100.0% | 14.1 / NA | 6 | F185F (TTC→TTT) | ascG | DNA‑binding transcriptional regulator |
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/6); total (0/6) |
CTGGTGGGGGAATCCATCGAGCCGGTAAGG‑AAAGCAATCTCCTGATGCCCGGCGTTTATCA > W3110S.gb/2837340‑2837400 | cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTtcatca < 1:1380689/62‑1 (MQ=255) cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa < 1:1015995/62‑1 (MQ=255) cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa < 1:1215628/62‑1 (MQ=255) cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa < 1:1512639/62‑1 (MQ=255) cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa < 1:1889357/62‑1 (MQ=255) cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa < 1:341162/62‑1 (MQ=255) | CTGGTGGGGGAATCCATCGAGCCGGTAAGG‑AAAGCAATCTCCTGATGCCCGGCGTTTATCA > W3110S.gb/2837340‑2837400 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |