Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,837,372 +A coding (552/1014 nt) ascG ← DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,837,3691.A100.0% 14.1 / NA 6F185F (TTC→TTTascGDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/6);  total (0/6)

CTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCA  >  W3110S.gb/2837340‑2837400
                              |                               
cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTtcatca  <  1:1380689/62‑1 (MQ=255)
cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa  <  1:1015995/62‑1 (MQ=255)
cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa  <  1:1215628/62‑1 (MQ=255)
cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa  <  1:1512639/62‑1 (MQ=255)
cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa  <  1:1889357/62‑1 (MQ=255)
cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa  <  1:341162/62‑1 (MQ=255)
                              |                               
CTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCA  >  W3110S.gb/2837340‑2837400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: