Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,550,367	+A	coding (299/834 nt)	fdhD ←	formate dehydrogenase formation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,550,364	1	.	A	100.0%	36.9 / NA	13	S101F (TCC→TTC)	fdhD	formate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/13);  total (0/13)

ACCCCATAAAGCGGCGGCTGG‑AAAGCTCAATTTGTACTTCAAGACCATTACAGGAAGGAA  >  W3110S.gb/3550344‑3550403                      |                                        aCCCCATAAAGCGGCGGCTGGAAAAGCTCAATTTGTACTTCAAGACCATTACAggaaggaa  <  1:1109624/61‑1 (MQ=255) aCCCCATAAAGCGGCGGCTGGAAAAGCTCAATTTGTACTTCAAGACCATTACAggaaggaa  <  1:1540818/61‑1 (MQ=255) aCCCCATAAAGCGGCGGCTGGAAAAGCTCAATTTGTACTTCAAGACCATTACAggaaggaa  <  1:1554835/61‑1 (MQ=255) aCCCCATAAAGCGGCGGCTGGAAAAGCTCAATTTGTACTTCAAGACCATTACAggaaggaa  <  1:1560141/61‑1 (MQ=255) aCCCCATAAAGCGGCGGCTGGAAAAGCTCAATTTGTACTTCAAGACCATTACAggaaggaa  <  1:1689064/61‑1 (MQ=255) aCCCCATAAAGCGGCGGCTGGAAAAGCTCAATTTGTACTTCAAGACCATTACAggaaggaa  <  1:1776317/61‑1 (MQ=255) aCCCCATAAAGCGGCGGCTGGAAAAGCTCAATTTGTACTTCAAGACCATTACAggaaggaa  <  1:2022088/61‑1 (MQ=255) aCCCCATAAAGCGGCGGCTGGAAAAGCTCAATTTGTACTTCAAGACCATTACAggaaggaa  <  1:2473847/61‑1 (MQ=255) aCCCCATAAAGCGGCGGCTGGAAAAGCTCAATTTGTACTTCAAGACCATTACAggaaggaa  <  1:273204/61‑1 (MQ=255) aCCCCATAAAGCGGCGGCTGGAAAAGCTCAATTTGTACTTCAAGACCATTACAggaaggaa  <  1:371603/61‑1 (MQ=255) aCCCCATAAAGCGGCGGCTGGAAAAGCTCAATTTGTACTTCAAGACCATTACAggaaggaa  <  1:684847/61‑1 (MQ=255) aCCCCATAAAGCGGCGGCTGGAAAAGCTCAATTTGTACTTCAAGACCATTACAggaaggaa  <  1:915845/61‑1 (MQ=255) aCCCCATAAAGCGGCGCCTGGAAAAGCTCAATTTGTACTTCAAGACCATTACAggaaggaa  <  1:524152/61‑1 (MQ=255)                      |                                        ACCCCATAAAGCGGCGGCTGG‑AAAGCTCAATTTGTACTTCAAGACCATTACAGGAAGGAA  >  W3110S.gb/3550344‑3550403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑