Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,669,364	+A	coding (901/1260 nt)	rho ←	transcription termination factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,669,361	1	.	A	100.0%	56.6 / NA	19	G302C (GGT→TGT)	rho	transcription termination factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (19/0);  total (19/0)

TGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACC‑AAAGAAGCGTTTCGGACGA  >  W3110S.gb/3669322‑3669380                                         |                    tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:2347874/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:975547/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:952763/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:783658/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:724386/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:492733/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:43684/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:2527356/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:2494121/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:1009304/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:2241544/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:201544/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:1673293/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:1663449/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:1649667/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:1565771/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:1561367/1‑60 (MQ=255) tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:1319440/1‑60 (MQ=255) tGGTAAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa  >  1:732991/1‑60 (MQ=255)                                         |                    TGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACC‑AAAGAAGCGTTTCGGACGA  >  W3110S.gb/3669322‑3669380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑