Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,862,940	(A)5→6	coding (79/1656 nt)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,862,935	1	.	A	100.0%	24.6 / NA	9	F28F (TTC→TTT)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/9);  total (0/9)

AAAGAAG‑AAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCCGGCG  >  W3110S.gb/3862929‑3862989        |                                                       aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg  <  1:1424699/62‑1 (MQ=255) aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg  <  1:1580027/62‑1 (MQ=255) aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg  <  1:1642716/62‑1 (MQ=255) aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg  <  1:2362793/62‑1 (MQ=255) aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg  <  1:2516661/62‑1 (MQ=255) aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg  <  1:2884334/62‑1 (MQ=255) aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg  <  1:550767/62‑1 (MQ=255) aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg  <  1:590655/62‑1 (MQ=255) aaaGAAGAAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg  <  1:900680/62‑1 (MQ=255)  aagaagaAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg  <  1:1141031/61‑1 (MQ=255)  aagaagaAAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCcggcg  <  1:141602/61‑1 (MQ=255)        |                                                       AAAGAAG‑AAAAACAGGATGGGAAGCGATGCTATCAGACTGGAAAGCCAGATATTCCCGGCG  >  W3110S.gb/3862929‑3862989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑