Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	216,887	(T)5→6	coding (117/825 nt)	yaeF ←	predicted lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	216,882	1	.	T	92.3%	34.3 / ‑2.4	13	coding (122/825 nt)	yaeF	predicted lipoprotein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (12/0);  total (13/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

GATGCTGGAATTTGACAGCCCAT‑G‑TTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTC  >  W3110S.gb/216859‑216919                          |                                      gATGCTGGAATTTGACAGCCCATCT‑TTTTTGCCTCGGCATCt                      >  1:1449637/1‑42 (MQ=37) gATGCTGGAATTTGACAGCCCAT‑GTTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTc  >  1:1083471/1‑62 (MQ=255) gATGCTGGAATTTGACAGCCCAT‑GTTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTc  >  1:1100743/1‑62 (MQ=255) gATGCTGGAATTTGACAGCCCAT‑GTTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTc  >  1:1109110/1‑62 (MQ=255) gATGCTGGAATTTGACAGCCCAT‑GTTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTc  >  1:1240275/1‑62 (MQ=255) gATGCTGGAATTTGACAGCCCAT‑GTTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTc  >  1:1530454/1‑62 (MQ=255) gATGCTGGAATTTGACAGCCCAT‑GTTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTc  >  1:1633101/1‑62 (MQ=255) gATGCTGGAATTTGACAGCCCAT‑GTTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTc  >  1:2245020/1‑62 (MQ=255) gATGCTGGAATTTGACAGCCCAT‑GTTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTc  >  1:2409785/1‑62 (MQ=255) gATGCTGGAATTTGACAGCCCAT‑GTTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTc  >  1:2507939/1‑62 (MQ=255) gATGCTGGAATTTGACAGCCCAT‑GTTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTc  >  1:2574824/1‑62 (MQ=255) gATGCTGGAATTTGACAGCCCAT‑GTTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTc  >  1:2676313/1‑62 (MQ=255) gATGCTGGAATTTGACAGCCCAT‑GTTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTc  >  1:888546/1‑62 (MQ=255)                          |                                      GATGCTGGAATTTGACAGCCCAT‑G‑TTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTC  >  W3110S.gb/216859‑216919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑