Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,260,617	+A	intergenic (+20/‑90)	dgkA → / → lexA	diacylglycerol kinase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,260,614	1	.	A	100.0%	17.5 / NA	7	intergenic (+17/‑93)	dgkA/lexA	diacylglycerol kinase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/7);  total (0/7)

CCGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑AAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTT  >  W3110S.gb/4260554‑4260659                                                              |                                              ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAt                                                >  1:63354/1‑61 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAt                                                >  1:2896994/1‑61 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:2108041/1‑62 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:81005/1‑62 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:366497/1‑62 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:2728730/1‑62 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:2628311/1‑62 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:2575127/1‑62 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:2218643/1‑62 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:2109259/1‑62 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:1623737/1‑62 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:1528330/1‑62 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:1463079/1‑62 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:1337151/1‑62 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:1241566/1‑62 (MQ=255) ccGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑a                                              >  1:1066996/1‑62 (MQ=255) ccGTGATTACCTGGTGCAATCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAt                                                >  1:1751555/1‑61 (MQ=255)                                              ccTTCCAGAATTCGATAAAATCTCTTGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt  <  1:1025761/62‑1 (MQ=255)                                              ccTTCCAGAATTCGATAAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt  <  1:1018610/62‑1 (MQ=255)                                              ccTTCCAGAATTCGATAAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt  <  1:1601249/62‑1 (MQ=255)                                              ccTTCCAGAATTCGATAAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt  <  1:2117892/62‑1 (MQ=255)                                              ccTTCCAGAATTCGATAAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt  <  1:1508733/62‑1 (MQ=255)                                              ccTTCCAGAATTCGATAAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt  <  1:1106725/62‑1 (MQ=255)                                              ccTTCCAGAATTCGATAAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCttt  <  1:780929/62‑1 (MQ=255)                                                              |                                              CCGTGATTACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGAT‑AAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTT  >  W3110S.gb/4260554‑4260659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑