Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,020,915	+A	coding (427/510 nt)	sulA ←	SOS cell division inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,020,912	1	.	A	100.0%	28.1 / NA	10	I144F (ATT→TTT)	sulA	SOS cell division inhibitor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/10);  total (0/10)

AGAGGATGCGCTTACCGGACGCATAAT‑AAACCCCATAGCGTTACCTTCATTTGCCG  >  W3110S.gb/1020886‑1020941                            |                              agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg  <  1:1055378/57‑1 (MQ=255) agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg  <  1:1485598/57‑1 (MQ=255) agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg  <  1:1504733/57‑1 (MQ=255) agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg  <  1:158305/57‑1 (MQ=255) agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg  <  1:2220826/57‑1 (MQ=255) agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg  <  1:2280881/57‑1 (MQ=255) agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg  <  1:466308/57‑1 (MQ=255) agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg  <  1:684135/57‑1 (MQ=255) agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg  <  1:686854/57‑1 (MQ=255)  gagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg  <  1:1940000/56‑1 (MQ=255)                       ataataAAACCCCATTCCGTTACCTTCATTTGCCg  <  1:2315346/35‑1 (MQ=12)                            |                              AGAGGATGCGCTTACCGGACGCATAAT‑AAACCCCATAGCGTTACCTTCATTTGCCG  >  W3110S.gb/1020886‑1020941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑