Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	3685380	3685427	48	14 [1]	[0] 23	ilvG	Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

TTTCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAACTG  >  W3110S.gb/3685428‑3685489 |                                                              tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:2149617/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:757727/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:402875/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:319123/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:2846165/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:2825545/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:2699242/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:2644729/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:2499656/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:2388931/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:2264100/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:217973/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:106578/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:2068520/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:2059207/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:1899136/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:1853478/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:1750812/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:1457/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:1306931/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:130288/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:1207031/62‑1 (MQ=255) tttCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAActg  <  1:1110175/62‑1 (MQ=255) |                                                              TTTCAGCGTACAGGTGGCAGGCATTTTTGTGGCAGCGAGAAATTCACGCAAAGCCGGAACTG  >  W3110S.gb/3685428‑3685489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑