Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	4112929	4112950	22	22 [0]	[0] 30	yrfF	predicted inner membrane protein

CGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCGG  >  W3110S.gb/4112951‑4113011 |                                                             cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGctg  >  1:876070/1‑59 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:257202/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:903161/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:902372/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:88288/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:526502/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:409950/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:404022/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:337863/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:2926877/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:2915717/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:2771029/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:2749493/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:2606502/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:1265901/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:2463921/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:2362999/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:2355009/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:233223/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:2215617/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:2066810/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:2045508/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:1888168/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:1826933/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:1673558/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:1620656/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:1536345/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCgg  >  1:132169/1‑61 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCg   >  1:2276324/1‑60 (MQ=255) cGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCg   >  1:57464/1‑60 (MQ=255) |                                                             CGGAAAGTTTTTTACTTCATCATGCAGCGAAAGATATCGTCCCTGACGCACTACATGGCGG  >  W3110S.gb/4112951‑4113011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑