| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,937,998 | 1 | . | A | 73.3% | 30.3 / 10.4 | 15 | coding (450/1005 nt) | dppF | dipeptide transporter |
| Reads supporting (aligned to +/- strand): ref base . (4/0); new base A (0/11); total (4/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGCCCTGTCGATGATGGCGAAAGTCGGCCTG‑AAAACCGAGCACTATGACCGCTATCCGCATATGTTCTCCGGCGGTCAGC > W3110S.gb/3937967‑3938047 | aaGCCCTGTCGATGATGGCGAAAGTCGGCCTGAAAAACCGAGCACTATGACCGCTATCCGCa < 1:122572/62‑1 (MQ=255)aaGCCCTGTCGATGATGGCGAAAGTCGGCCTGAAAAACCGAGCACTATGACCGCTATCCGCa < 1:1491551/62‑1 (MQ=255)aaGCCCTGTCGATGATGGCGAAAGTCGGCCTGAAAAACCGAGCACTATGACCGCTATCCGCa < 1:2026864/62‑1 (MQ=255)aaGCCCTGTCGATGATGGCGAAAGTCGGCCTGAAAAACCGAGCACTATGACCGCTATCCGCa < 1:2038187/62‑1 (MQ=255)aaGCCCTGTCGATGATGGCGAAAGTCGGCCTGAAAAACCGAGCACTATGACCGCTATCCGCa < 1:2169605/62‑1 (MQ=255)aaGCCCTGTCGATGATGGCGAAAGTCGGCCTGAAAAACCGAGCACTATGACCGCTATCCGCa < 1:2209311/62‑1 (MQ=255)aaGCCCTGTCGATGATGGCGAAAGTCGGCCTGAAAAACCGAGCACTATGACCGCTATCCGCa < 1:410473/62‑1 (MQ=255)aaGCCCTGTCGATGATGGCGAAAGTCGGCCTGAAAAACCGAGCACTATGACCGCTATCCGCa < 1:658770/62‑1 (MQ=255)aaGCCCTGTCGATGATGGCGAAAGTCGGCCTGAAAAACCGAGCACTATGACCGCTATCCGCa < 1:714138/62‑1 (MQ=255)aaGCCCTGTCGATGATGGCGAAAGTCGGCCTGAAAAACCGAGCACTATGACCGCTATCCGCa < 1:763909/62‑1 (MQ=255)aaGCCCTGTCGATGATGGCGAAAGTCGGCCTGAAAAACCGAGCACTATGACCGCTATCCGCa < 1:952748/62‑1 (MQ=255) aaaGTCGGCCTG‑AAAACCGAGCACTATGACCGCTATCCGCATATGTTCTCCGGCGgtcagc > 1:1689847/1‑61 (MQ=255) aaaGTCGGCCTG‑AAAACCGAGCACTATGACCGCTATCCGCATATGTTCTCCGGCGgtcagc > 1:2410509/1‑61 (MQ=255) aaaGTCGGCCTG‑AAAACCGAGCACTATGACCGCTATCCGCATATGTTCTCCGGCGgtcagc > 1:244901/1‑61 (MQ=255) aaaGTCGGCCTG‑AAAACCGAGCACTATGACCGCTATCCGCATATGTTCTCCGGCGgtcagc > 1:2701695/1‑61 (MQ=255) | AAGCCCTGTCGATGATGGCGAAAGTCGGCCTG‑AAAACCGAGCACTATGACCGCTATCCGCATATGTTCTCCGGCGGTCAGC > W3110S.gb/3937967‑3938047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |