| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 36,385 | 0 | A | G | 73.1% | 77.4 / 30.4 | 52 | F485F (TTT→TTC) | caiC | predicted crotonobetaine CoA ligase:carnitine CoA ligase |
| Reads supporting (aligned to +/- strand): ref base A (14/0); new base G (38/0); total (52/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCTTTG > W3110S.gb/36324‑36436 | ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:1076845/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:82851/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:779898/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:512646/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:322912/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:2897304/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:2777424/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:2767212/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:2387148/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:2090216/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:1994454/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:1888159/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:1850547/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:1768569/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:1230921/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGaaa > 1:1574467/1‑62 (MQ=255)ttCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCG‑AGAAGCGGaaaa > 1:2712599/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAAtt > 1:1683625/1‑51 (MQ=37) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCtt > 1:2822151/1‑57 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:2663633/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:576343/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:2693807/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:2704337/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:2725915/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:1444201/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:1388225/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:2802156/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:2804155/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:753160/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:710857/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:133231/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:707867/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:384785/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:697895/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:432426/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:4673/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:697402/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:75740/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:1710758/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:1680754/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:1670311/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:1990423/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:823014/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:206821/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:2077663/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:790526/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:1129408/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:2237087/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:2323084/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:2330280/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:1526521/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:2504066/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc > 1:2613506/1‑62 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTACACTTCGCt > 1:1529667/1‑61 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATACCTCTTCGCTc > 1:2047356/1‑62 (MQ=255) aaaTTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCtt > 1:2104418/1‑62 (MQ=255) cAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCttt > 1:802930/1‑62 (MQ=255) cAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCttt > 1:1074235/1‑62 (MQ=255) cAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCttt > 1:501008/1‑62 (MQ=255) cAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCttt > 1:1289977/1‑62 (MQ=255) cAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCttt > 1:2853405/1‑62 (MQ=255) cAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCttt > 1:2841133/1‑62 (MQ=255) cAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCttt > 1:2554089/1‑62 (MQ=255) cAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCttt > 1:2210863/1‑62 (MQ=255) cAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCttt > 1:2090086/1‑62 (MQ=255) cAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCttt > 1:1998806/1‑62 (MQ=255) cAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCttt > 1:1557126/1‑62 (MQ=255) cAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCttt > 1:1694404/1‑62 (MQ=255) cAGAAGCGGAAAAATTCCTCTTCGCT‑AATGTTTCACCTTCATTCAGCACCACAAATGCTTTg > 1:409777/1‑62 (MQ=255) | TTCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCTTTG > W3110S.gb/36324‑36436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |