| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 486,797 | 0 | T | A | 72.5% | 105.2 / 51.4 | 69 | S346S (TCT→TCA) | kefA | fused mechanosensitive channel proteins |
| Reads supporting (aligned to +/- strand): ref base T (12/7); new base A (50/0); total (62/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGA > W3110S.gb/486761‑486855 | gCAGATTGCCTTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACaaa > 1:32144/1‑62 (MQ=255)gCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACaaa > 1:1005107/1‑62 (MQ=255)gCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACaaa > 1:2926466/1‑62 (MQ=255)gCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACaaa > 1:547985/1‑62 (MQ=255)gCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACaaa > 1:585375/1‑62 (MQ=255)gCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACaaa > 1:1663614/1‑62 (MQ=255)gCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACaaa > 1:1587723/1‑62 (MQ=255)gCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACaaa > 1:1354128/1‑62 (MQ=255)gCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACaaa > 1:1289050/1‑62 (MQ=255)gCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACaaa > 1:809879/1‑62 (MQ=255)gCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACaaa > 1:827800/1‑62 (MQ=255)gCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACaaa > 1:2290987/1‑62 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACaaa > 1:600309/1‑37 (MQ=37) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGa > 1:1855020/1‑56 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2425898/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2385515/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2601543/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2278927/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2724623/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2759978/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2884081/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2188077/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2205327/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:364425/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:386113/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:392385/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:420020/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:503862/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:508112/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:542518/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:587652/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:665059/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:845169/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:904239/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:911840/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:948094/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1997249/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1871057/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1882546/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1798240/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1894827/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1910216/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1927762/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1937820/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1946007/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:195024/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1963184/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1965338/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1227499/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1217898/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1213648/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2089688/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2095060/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1188783/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2161700/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2175270/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:1184895/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTgg > 1:2190367/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTg > 1:976194/1‑60 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTCGGCAGATGAACTg > 1:1867712/1‑60 (MQ=255) ctgctgTTGTCACGTATCCTGTACCAGCAACAACAAACACTCCCCTAGGCAGATGAACTgg > 1:540106/1‑61 (MQ=255) ctgctgTTGTCACGTATCCTCTACCAGCAACAACAAACACTCCCCTCGGCAGATGAAc > 1:20110/1‑58 (MQ=255) gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:1564645/62‑1 (MQ=255) gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:1556384/62‑1 (MQ=255) gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:69363/62‑1 (MQ=255) gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:2787516/62‑1 (MQ=255) gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:21047/62‑1 (MQ=255) gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:2187547/62‑1 (MQ=255) gtCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:100084/62‑1 (MQ=255) tctcGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:1261116/61‑1 (MQ=255) tctcGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGa < 1:2742204/61‑1 (MQ=255) | GCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGA > W3110S.gb/486761‑486855 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |