| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 823,115 | 0 | T | C | 73.2% | 86.6 / 33.0 | 56 | E349E (GAA→GAG) | ybhO | cardiolipin synthase 2 |
| Reads supporting (aligned to +/- strand): ref base T (15/0); new base C (41/0); total (56/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCA > W3110S.gb/823094‑823167 | ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCt > 1:2186523/1‑43 (MQ=37)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2828466/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2378907/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2407161/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2419685/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2534997/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:261534/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:265086/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2725442/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2745815/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2796739/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2822483/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:244088/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:373653/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:413371/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:565165/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:588464/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:622361/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:656934/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:683079/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:696260/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:79582/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:852255/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:98229/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:173992/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:1099867/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:1246069/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:1259429/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:1282589/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:1401421/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:1511115/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:1658280/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:1680350/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:1988518/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2035857/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2241980/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2235324/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2045604/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2101445/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGCGGCGATAATGCCg > 1:2124635/1‑58 (MQ=255)ggTGCGTTTCGGCAGCATGGTCTCATCGACCTGCTGACAATCTGAGGCGATAATGCCg > 1:2679325/1‑58 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:2536411/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:892239/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:858698/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:1263809/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:1096699/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:1320858/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:1488478/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:526128/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:484827/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:232714/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:1803978/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:2659306/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:279225/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg > 1:2013952/1‑60 (MQ=255) ggCAGCATGGTTTCATCCACCTGCTGACAATCT‑‑GGCAATAATGCCGTTCAGATTATCGCGCa > 1:2411227/1‑62 (MQ=255) | GGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCA > W3110S.gb/823094‑823167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |