| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,487,144 | 0 | T | G | 74.3% | 194.7 / 60.0 | 101 | V771V (GTT→GTG) | hrpA | ATP‑dependent helicase |
| Reads supporting (aligned to +/- strand): ref base T (11/15); new base G (0/75); total (11/90) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.55e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 101 total aligned reads displayed.
GTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACG > W3110S.gb/1487100‑1487190 | gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt < 1:1011356/62‑1 (MQ=255)gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt < 1:401868/62‑1 (MQ=255)gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt < 1:193982/62‑1 (MQ=255)gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt < 1:2909577/62‑1 (MQ=255)gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt < 1:28376/62‑1 (MQ=255)gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt < 1:1344078/62‑1 (MQ=255)gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt < 1:724671/62‑1 (MQ=255)gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt < 1:282499/62‑1 (MQ=255)gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt < 1:2763624/62‑1 (MQ=255)gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt < 1:945291/62‑1 (MQ=255)gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt < 1:2521161/62‑1 (MQ=255)gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt < 1:1170582/62‑1 (MQ=255) tAGAAGAGCTGGAACACAAATCACGTCGCCGCTATATTCTGGTTGATGACGAAACgttgtt < 1:1382613/61‑1 (MQ=255) tAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt < 1:2267340/61‑1 (MQ=255) ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCAc > 1:163988/1‑61 (MQ=255) ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCAc > 1:1351480/1‑61 (MQ=255) ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg > 1:1165711/1‑62 (MQ=255) ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg > 1:1980020/1‑62 (MQ=255) ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg > 1:852370/1‑62 (MQ=255) ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg > 1:2450074/1‑62 (MQ=255) ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg > 1:2305173/1‑62 (MQ=255) ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg > 1:1918795/1‑62 (MQ=255) ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg > 1:388341/1‑62 (MQ=255) ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg > 1:1689102/1‑62 (MQ=255) ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg > 1:833022/1‑62 (MQ=255) gcgATATTCTGGTGGATGACGAAATGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2514769/60‑1 (MQ=255) gcgATATTCTGGTGGATGACGAAACGCTGTTTTAGTTCTACGACCAGCGCATCAGCCACg < 1:1478933/60‑1 (MQ=38) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2572/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:284388/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:67269/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:787815/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2803382/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2770449/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:806696/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2625282/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2881912/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:824551/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:840604/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2538113/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2526385/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:873251/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:89551/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1021024/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:47054/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:652721/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:54255/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2920533/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:541765/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:322112/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:329785/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:334404/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:374056/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:52738/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:401752/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:485960/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:404970/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:432364/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:464119/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1331408/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2495815/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1713246/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1698288/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:169026/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:155572/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1553470/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1498270/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1489133/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1479245/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1359086/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1740574/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1308458/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1280982/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1280761/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1240628/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1225707/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1210969/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:120027/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1125312/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1098756/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2006109/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2409001/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2338509/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2338063/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2234000/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2206833/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2204869/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2173755/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2130866/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2031554/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1785751/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1981405/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:196483/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1921213/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1902830/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1882845/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1822211/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1802689/60‑1 (MQ=39) gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1799279/60‑1 (MQ=39) cgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:1370393/59‑1 (MQ=39) gATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg < 1:2249097/58‑1 (MQ=39) | GTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACG > W3110S.gb/1487100‑1487190 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |