| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,007,769 | 0 | A | G | 74.0% | 171.5 / 63.1 | 104 | E110E (GAA→GAG) | fliS | flagellar protein potentiates polymerization |
| Reads supporting (aligned to +/- strand): ref base A (27/0); new base G (0/77); total (27/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.53e-25 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 117 total aligned reads displayed.
GCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGTTACTCTCCCC > W3110S.gb/2007721‑2007825 | gcgcTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTaa > 1:2635012/1‑62 (MQ=255)gcgcTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTaa > 1:2375957/1‑62 (MQ=255)gcgcTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTaa > 1:1703872/1‑62 (MQ=255)gcgcTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTaa > 1:512777/1‑62 (MQ=255)gcgcTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTaa > 1:1262983/1‑62 (MQ=255)gcgcTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTaa > 1:1229024/1‑62 (MQ=255)gcgcTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTaa > 1:1931300/1‑62 (MQ=255)gcgcTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTaa > 1:698717/1‑62 (MQ=255)gcgcTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTaa > 1:1623061/1‑62 (MQ=255)gcgcTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTaa > 1:2034422/1‑62 (MQ=255) aaGCCTATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1220739/61‑1 (MQ=255) aggcAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1465719/57‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAATCATTAATGCGTAatat < 1:589390/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGTATTAATGCGTAatat < 1:2372370/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCGTTAATGCGTAatat < 1:1977321/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2600178/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2744796/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2646300/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2694184/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:287121/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2492864/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2406159/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2305763/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2108612/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:947062/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2055599/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2033862/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:49757/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:876548/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:764651/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:760277/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:743185/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:605086/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:561554/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:531072/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:501759/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2799769/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:491183/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:385096/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:372021/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:326171/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:296910/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:974375/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2843527/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2808161/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1309606/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1641064/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1621196/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1584927/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:157018/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1511230/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1502705/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1451870/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1411277/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1993794/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1237587/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1214832/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1212657/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1190364/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1127286/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1118608/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:111394/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1711972/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1878065/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1785147/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:962880/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:193093/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1726312/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2020790/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:967626/60‑1 (MQ=255) aGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGCAatat < 1:1875042/60‑1 (MQ=255) gCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:753272/59‑1 (MQ=255) gCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2360074/59‑1 (MQ=255) aaTTTACGCAACGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2298140/56‑1 (MQ=255) cGATGTCTCCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:2756414/45‑1 (MQ=255) cGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCTCAATATTGCCGATGCCTGGAAag > 1:1627468/1‑62 (MQ=255) cGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGaa > 1:833124/1‑60 (MQ=255) cGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAag > 1:1002871/1‑62 (MQ=255) cGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAag > 1:1662989/1‑62 (MQ=255) cGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAag > 1:511621/1‑62 (MQ=255) cGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAag > 1:1340671/1‑62 (MQ=255) cGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAag > 1:1514652/1‑62 (MQ=255) cGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAag > 1:1746627/1‑62 (MQ=255) cGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAag > 1:2825140/1‑62 (MQ=255) cGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAag > 1:1649668/1‑62 (MQ=255) ctcCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCg > 1:2247651/1‑61 (MQ=255) ctcCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGt > 1:1935422/1‑62 (MQ=255) tcCGCAGTCGAAGAGGTGGAAGCATTAATGCGTAatat < 1:1105115/38‑1 (MQ=38) gaagaaGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGTTActct > 1:2159450/1‑58 (MQ=255) gaagaaGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGTTACtctc > 1:872763/1‑59 (MQ=255) gaagaaGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGTTACTCTcccc > 1:1542222/1‑62 (MQ=255) gaagaaGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGTTACTCTcccc > 1:2444306/1‑62 (MQ=255) gaagaaGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGTTACTCTccc > 1:917565/1‑61 (MQ=255) gaagaaGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGTTACTCTccc > 1:212659/1‑61 (MQ=255) gaagaaGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGTTACTCTccc > 1:799636/1‑61 (MQ=255) gaagaaGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGTTACTCTccc > 1:2107793/1‑61 (MQ=255) gaagaaGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGTTACTCTccc > 1:1890145/1‑61 (MQ=255) gaagaaGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGTTACTCTccc > 1:1714548/1‑61 (MQ=255) gaagaaGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGTTACTCTccc > 1:1004/1‑61 (MQ=255) gaagaaGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGCAAAGAGTCGTTAc > 1:2775415/1‑55 (MQ=255) | GCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATATTGCCGATGCCTGGAAAGAGTCGTTACTCTCCCC > W3110S.gb/2007721‑2007825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |