| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,118,857 | 0 | G | T | 72.9% | 144.6 / 130.2 | 151 | L136L (CTC→CTA) | wcaL | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base G (24/17); new base T (110/0); total (134/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 151 total aligned reads displayed.
ATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGG > W3110S.gb/2118803‑2118909 | atCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtg < 1:934403/62‑1 (MQ=255)atCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtg < 1:493011/62‑1 (MQ=255)atCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtg < 1:1225499/62‑1 (MQ=255)atCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtg < 1:528722/62‑1 (MQ=255)atCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtg < 1:129070/62‑1 (MQ=255)atCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtg < 1:753839/62‑1 (MQ=255) tCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtg < 1:2811781/61‑1 (MQ=255) tCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtg < 1:2138488/61‑1 (MQ=255) cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGtt > 1:2685953/1‑62 (MQ=255) cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGtt > 1:1731544/1‑62 (MQ=255) cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGtt > 1:472814/1‑62 (MQ=255) cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGtt > 1:2834997/1‑62 (MQ=255) cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGtt > 1:593097/1‑62 (MQ=255) cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGtt > 1:1778102/1‑62 (MQ=255) cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGtt > 1:292803/1‑62 (MQ=255) cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGtt > 1:1001477/1‑62 (MQ=255) cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGtt > 1:1885314/1‑62 (MQ=255) cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGtt > 1:204421/1‑62 (MQ=255) cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGtt > 1:1244762/1‑62 (MQ=255) cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGtt > 1:2516284/1‑62 (MQ=255) cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGtt > 1:2837713/1‑62 (MQ=255) tGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCaa > 1:1867792/1‑57 (MQ=255) tGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCaa > 1:1356050/1‑57 (MQ=255) tGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCaa > 1:584444/1‑57 (MQ=255) tGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCaa > 1:2403438/1‑57 (MQ=255) tGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCaa > 1:1169225/1‑57 (MQ=255) tGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCaa > 1:1134908/1‑57 (MQ=255) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATc > 1:570492/1‑60 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATc > 1:1198162/1‑60 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2735621/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2825899/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2776017/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2773014/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:42125/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2731926/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2695530/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2612372/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2592414/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:246144/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:647844/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:960693/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:896684/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:865345/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:846274/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:810667/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:745915/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:705266/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:662153/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2870053/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:617595/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:567811/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:472324/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2292840/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:347880/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:343727/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:313342/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2899332/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1442594/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2443908/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:16854/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1603597/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1582235/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1576110/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:153035/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1510919/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1480127/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1454827/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1694089/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1424336/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1410290/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1382174/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1380012/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1304149/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1188629/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1132688/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1032882/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2263814/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2237114/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2159207/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2146894/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2117471/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2103823/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:209795/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2078726/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2024888/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1949112/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1885439/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2349285/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1833232/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:235100/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1777929/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:240135/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1703445/1‑62 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCg > 1:383976/1‑61 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCg > 1:1766630/1‑61 (MQ=25) cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACAAATCgg > 1:1539990/1‑62 (MQ=21) cAGTTCGCGTAGTTTTGCCGCGGTTACCACAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:774940/1‑62 (MQ=21) cAGTTCGCGTAGTTTTGCCGCGGTTACACCAGCAGGGCCAAAATGAGCGAt > 1:1645185/1‑51 (MQ=255) cAGTTCGCGTAGTTTTGCAGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:2438659/1‑62 (MQ=25) cAGTTAGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg > 1:1601514/1‑62 (MQ=21) | ATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGG > W3110S.gb/2118803‑2118909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |