| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,696,454 | 0 | T | C | 74.3% | 123.9 / 40.8 | 74 | K31K (AAA→AAG) | tadA | tRNA‑specific adenosine deaminase |
| Reads supporting (aligned to +/- strand): ref base T (0/19); new base C (0/55); total (0/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTATGCACTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTC > W3110S.gb/2696403‑2696489 | gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:333708/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2325188/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2535078/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2601526/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2664761/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1012655/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:270952/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2729001/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2737392/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2748324/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2808275/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2901232/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2926692/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:297533/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:298746/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:314999/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2688036/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:360934/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:37283/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:412464/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:462322/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:526571/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:554167/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:581574/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:594584/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:721248/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:723494/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:799906/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:809839/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:945668/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:9523/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1889393/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1489346/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1586003/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1199045/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2218525/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1697500/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:174724/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1810429/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1838780/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1211324/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1480160/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1940084/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1416699/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2104292/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2119547/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:2159204/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1372126/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1079346/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1232964/62‑1 (MQ=255)gTTATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTAGCCAGCg < 1:1754843/62‑1 (MQ=255) ttATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:192229/61‑1 (MQ=255) ttATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:285029/61‑1 (MQ=255) ttATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:1127268/61‑1 (MQ=255) tATGCACTAATACCGCGCCGACCGGTACTTCCCGCTCATCCCAGGCACGCTTCGCCAGCg < 1:656232/60‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:742528/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:1651095/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:1113865/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:818276/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:900388/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:917408/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:1046768/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:47464/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:1594989/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:2916543/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:1867962/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:2790391/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:2013976/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:2153281/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:2179486/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:2368944/61‑1 (MQ=255) cTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:2335935/61‑1 (MQ=255) tAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAgcgc < 1:564578/60‑1 (MQ=255) gCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTc < 1:794548/61‑1 (MQ=255) | GTTATGCACTAATACCGCGCCGACCGGCACTTCCCGCTCATCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTC > W3110S.gb/2696403‑2696489 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |