Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,186,293	1	.	T	73.3%	30.8 / 10.0	15	coding (238/2466 nt)	yqiG	predicted outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base . (4/0);  new base T (11/0);  total (15/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ACCTCCAATGGGTATTAATTGATAACCAAACTGCAG‑TTTGCGTGACACCAGAGCAATTAACATTATTAGGATTTACTGATGAA  >  W3110S.gb/3186258‑3186340                                     |                                                aCCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTTGCGTGACACCAGAGCAATTAAc                        >  1:1176900/1‑62 (MQ=255) aCCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTTGCGTGACACCAGAGCAATTAAc                        >  1:12747/1‑62 (MQ=255) aCCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTTGCGTGACACCAGAGCAATTAAc                        >  1:1688656/1‑62 (MQ=255) aCCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTTGCGTGACACCAGAGCAATTAAc                        >  1:2378778/1‑62 (MQ=255) aCCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTTGCGTGACACCAGAGCAATTAAc                        >  1:2671469/1‑62 (MQ=255) aCCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTTGCGTGACACCAGAGCAATTAAc                        >  1:279429/1‑62 (MQ=255) aCCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTTGCGTGACACCAGAGCAATTAAc                        >  1:2918020/1‑62 (MQ=255) aCCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTTGCGTGACACCAGAGCAATTAAc                        >  1:2922409/1‑62 (MQ=255) aCCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTTGCGTGACACCAGAGCAATTAAc                        >  1:571838/1‑62 (MQ=255) aCCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTTGCGTGACACCAGAGCAATTAAc                        >  1:968640/1‑62 (MQ=255) aCCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTTGCGTGACACCAGAGAAATTAAc                        >  1:1310056/1‑62 (MQ=255)                      aTAACCAAACTGCAG‑TTTGCGTGACACCAGAGCAATTAACATTATTAGGATTTACTGATGaa  >  1:1738178/1‑62 (MQ=255)                      aTAACCAAACTGCAG‑TTTGCGTGACACCAGAGCAATTAACATTATTAGGATTTACTGATGaa  >  1:2771437/1‑62 (MQ=255)                      aTAACCAAACTGCAG‑TTTGCGTGACACCAGAGCAATTAACATTATTAGGATTTACTGATGaa  >  1:307709/1‑62 (MQ=255)                      aTAACCAAACTGCAG‑TTTGCGTGACACCAGAGCAATTAACATTATTAGGATTTACTGATGaa  >  1:672044/1‑62 (MQ=255)                                     |                                                ACCTCCAATGGGTATTAATTGATAACCAAACTGCAG‑TTTGCGTGACACCAGAGCAATTAACATTATTAGGATTTACTGATGAA  >  W3110S.gb/3186258‑3186340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑