| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,665 | A→C | V443V (GTA→GTC) | thrA → | fused aspartokinase I and homoserine dehydrogenase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,665 | 0 | A | C | 82.8% | 280.2 / 33.2 | 99 | V443V (GTA→GTC) | thrA | fused aspartokinase I and homoserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base A (17/0); new base C (0/82); total (17/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-19 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
CCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAT > W3110S.gb/1604‑1719 | ccAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:1354435/62‑1 (MQ=255)ccAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:2128492/62‑1 (MQ=255)ccAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:2842832/62‑1 (MQ=255)ccAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:2107469/62‑1 (MQ=255)ccAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:1592642/62‑1 (MQ=255)ccAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:388963/62‑1 (MQ=255)ccAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:202902/62‑1 (MQ=255)ccAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:616074/62‑1 (MQ=255)ccAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:1997902/62‑1 (MQ=255)ccAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:1301750/62‑1 (MQ=255)ccAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:2384458/62‑1 (MQ=255)ccAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:1114769/62‑1 (MQ=255) cAATATCAACATTGTTGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:722683/61‑1 (MQ=255) cAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTa < 1:2714099/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCTTGGTCAATAACGATGATGCGACCACTGGCg < 1:2590095/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTTGTCAATAACGATGATGCGACCACTGGCg < 1:1988850/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTggtg < 1:1039749/61‑3 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGTCg < 1:32970/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGTCg < 1:2710764/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2915508/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:288125/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:248168/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2871356/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2830291/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2816856/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2799283/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2760730/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2721040/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2626805/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2496531/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:926024/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:565603/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:912350/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:870261/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:771620/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:743738/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:70931/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:701235/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:694591/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:68997/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:596417/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:580677/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2916315/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:521634/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:519683/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:515185/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:474235/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:428377/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:414651/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:38529/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:350595/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:322085/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1428062/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1860892/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2427514/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1762918/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1742514/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:172317/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1692879/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1595475/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1577034/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:153525/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1808382/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1411345/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1339860/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1320920/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1288559/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1192200/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1182138/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1173803/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:113603/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1134288/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1887358/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2420271/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2272607/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2248699/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2236887/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2224092/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2220424/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2216050/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2192222/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2176726/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1879566/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:2140562/61‑1 (MQ=255) cAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:1941132/61‑1 (MQ=255) aGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:288791/60‑1 (MQ=255) ttctGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCg < 1:212787/53‑1 (MQ=255) tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCa > 1:1510131/1‑60 (MQ=255) tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCGAt > 1:2076397/1‑62 (MQ=255) tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAt > 1:1764662/1‑62 (MQ=255) tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAt > 1:917472/1‑62 (MQ=255) tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAt > 1:2305410/1‑62 (MQ=255) tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAt > 1:1845175/1‑62 (MQ=255) tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAt > 1:801705/1‑62 (MQ=255) tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAt > 1:1822219/1‑62 (MQ=255) tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAt > 1:753612/1‑62 (MQ=255) tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAt > 1:2783910/1‑62 (MQ=255) tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAt > 1:2787500/1‑62 (MQ=255) tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAt > 1:1485782/1‑62 (MQ=255) tCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAt > 1:2847719/1‑62 (MQ=255) | CCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAT > W3110S.gb/1604‑1719 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |