| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 52,880 | T→G | A179A (GCA→GCC) | pdxA ← | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 52,880 | 0 | T | G | 87.5% | 143.4 / 7.9 | 48 | A179A (GCA→GCC) | pdxA | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (6/0); new base G (0/42); total (6/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.15e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01 | |||||||||||
ATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCGTCTGCGATATCGCGCAGCGGTAAATGCGTCGTTGCCAGC > W3110S.gb/52850‑52934 | aTGCAAAATAGCAATCAGTTCGTGCAAAAGGGCAgg < 1:2492339/36‑1 (MQ=37)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:2768591/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:227049/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1008765/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:2317189/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:2394850/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:2491863/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:2571336/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:2613852/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:2652131/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:2673100/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:227619/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:282560/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:2899291/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:377071/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:381936/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:421127/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:668531/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:67198/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:718613/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:734445/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:968280/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1630042/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1212983/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1300350/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1203472/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1345401/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1391202/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1430498/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1506289/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1605801/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:2221033/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1608515/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1754285/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1796028/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1966744/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:2012471/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1118077/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:2027279/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:2184316/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGTGCAAAAGGGCAgg < 1:1056992/36‑1 (MQ=255)aTGCAAAATAGCAATCACTTCGAGCAAAAGGGCAgg < 1:1707075/36‑1 (MQ=38) gCAAAAGTGCAGGGGTGATAGCGTCTGCGATATCGCGCAGCGGTAAATGCGTCGTTGCCAgc > 1:273648/1‑62 (MQ=255) gCAAAAGTGCAGGGGTGATAGCGTCTGCGATATCGCGCAGCGGTAAATGCGTCGTTGCCAgc > 1:724840/1‑62 (MQ=255) gCAAAAGTGCAGGGGTGATAGCGTCTGCGATATCGCGCAGCGGTAAATGCGTCGTTGCCAgc > 1:1302027/1‑62 (MQ=255) gCAAAAGTGCAGGGGTGATAGCGTCTGCGATATCGCGCAGCGGTAAATGCGTCGTTGCCAgc > 1:2536287/1‑62 (MQ=255) gCAAAAGTGCAGGGGTGATAGCGTCTGCGATATCGCGCAGCGGTAAATGCGTCGTTGCCAgc > 1:2185087/1‑62 (MQ=255) gCAAAAGTGCAGGGGTGATAGCGTCTGCGATATCGCGCAGCGGTAAATGCGTCGTTGCCAg > 1:235218/1‑61 (MQ=255) | ATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCGTCTGCGATATCGCGCAGCGGTAAATGCGTCGTTGCCAGC > W3110S.gb/52850‑52934 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |