Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,093,686	T→C	V130A (GTA→GCA)	ycdT →	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,093,686	0	T	C	100.0%	56.3 / NA	18	V130A (GTA→GCA)	ycdT	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/0);  total (18/0)

ATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATT  >  W3110S.gb/1093641‑1093731                                              |                                              attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                               >  1:1166114/1‑62 (MQ=255) attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                               >  1:964932/1‑62 (MQ=255) attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                               >  1:957788/1‑62 (MQ=255) attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                               >  1:898412/1‑62 (MQ=255) attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                               >  1:843837/1‑62 (MQ=255) attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                               >  1:454216/1‑62 (MQ=255) attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                               >  1:2924779/1‑62 (MQ=255) attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                               >  1:2457660/1‑62 (MQ=255) attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                               >  1:2157685/1‑62 (MQ=255) attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                               >  1:1713707/1‑62 (MQ=255) attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                               >  1:1393688/1‑62 (MQ=255)                           tgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt     >  1:2468778/1‑62 (MQ=255)                              atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgcg           >  1:1628103/1‑53 (MQ=255)                              atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAg             >  1:2415267/1‑51 (MQ=255)                              atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttatt  >  1:3839/1‑62 (MQ=255)                              atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttatt  >  1:525005/1‑62 (MQ=255)                              atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttatt  >  1:156911/1‑62 (MQ=255)                              atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttatt  >  1:1046366/1‑62 (MQ=255)                                              |                                              ATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATT  >  W3110S.gb/1093641‑1093731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑