Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	62,059	C→T	E402E (GAG→GAA)	hepA ←	RNA polymerase‑associated helicase protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	62,059	0	C	T	100.0%	52.5 / NA	18	E402E (GAG→GAA)	hepA	RNA polymerase‑associated helicase protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/18);  total (0/18)

GTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTG  >  W3110S.gb/62003‑62064                                                         |      gtgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:925066/62‑1 (MQ=255) gtgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:781452/62‑1 (MQ=255) gtgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:489794/62‑1 (MQ=255) gtgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:1646826/62‑1 (MQ=255) gtgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:308077/62‑1 (MQ=255) gtgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:2904701/62‑1 (MQ=255) gtgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:2080311/62‑1 (MQ=255) gtgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:2120008/62‑1 (MQ=255) gtgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:2866907/62‑1 (MQ=255) gtgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:2684338/62‑1 (MQ=255)  tgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:1401529/61‑1 (MQ=255)  tgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:2384486/61‑1 (MQ=255)  tgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:1781064/61‑1 (MQ=255)  tgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:1732657/61‑1 (MQ=255)  tgtTACGGAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:1555695/61‑1 (MQ=255)      aCGTAACAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:1428285/57‑1 (MQ=255)           aCAGCACGCGGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:980588/52‑1 (MQ=39)                 cgcgGCTGGTACCGTGGCGATCCATCAGCATCGAAACCAGTTCCTg  <  1:1160760/46‑1 (MQ=38)                                                         |      GTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTG  >  W3110S.gb/62003‑62064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑