Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,320,969	T→C	G177G (GGA→GGG)	trpC ←	fused indole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,320,969	0	T	C	100.0%	211.8 / NA	63	G177G (GGA→GGG)	trpC	fused indole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/63);  total (0/63)

TGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTC  >  W3110S.gb/1320966‑1321013    |                                             tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2880760/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2424872/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2475583/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:252250/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:253694/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2573864/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2649953/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2671588/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2675152/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2681431/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2685445/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:269141/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2705170/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2728529/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2763223/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2799007/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:241243/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:322830/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:405669/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:464355/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:470937/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:484409/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:486376/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:689764/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:734641/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:762157/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:793946/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:799117/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:873340/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:892437/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:947366/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:963694/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1800356/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1032647/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:105386/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1057132/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1063896/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1065289/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1157537/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1175015/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1196549/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1241628/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1270090/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1323452/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1402808/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1489451/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:163095/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1710082/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:101292/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1914146/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:1930793/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2087606/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2103321/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2121052/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2132089/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2135309/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2247982/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2268871/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2352457/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2387261/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACTGACTTc  <  1:2398151/48‑1 (MQ=38) tGCCCCCAATGCAATGGCGCGCTCCAGTTCCTCTTCATTACCGACTTc  <  1:1997561/48‑1 (MQ=37) tGCCCCCAATGCAATGGCGCGCGCCAGTTCCTCTTCATTACTGACTTc  <  1:2004215/48‑1 (MQ=37)    |                                             TGCTCCCAATGCAATGGCGCGCTCCTGTTCCTCTTCATTACTGACTTC  >  W3110S.gb/1320966‑1321013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑