Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,358,426	G→A	R133R (CGC→CGT)	sapA ←	predicted antimicrobial peptide transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,358,426	0	G	A	100.0%	56.7 / NA	19	R133R (CGC→CGT)	sapA	predicted antimicrobial peptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/1);  total (18/1)

TATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTC  >  W3110S.gb/1358410‑1358471                 |                                              tATGCCACGGGTTGTTACGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGtattc  <  1:2015333/62‑1 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:1944924/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:815113/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:660339/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:642703/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:510275/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:338174/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:2919392/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:2481412/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:2429979/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:1002503/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:1892134/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:1768441/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:1740132/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:1673105/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:1612339/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:1356709/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:1317231/1‑38 (MQ=255)         gggTTGTTACGGTCAAAAATTCGCTGGAAGGTAAacac                  >  1:1116371/1‑38 (MQ=255)                 |                                              TATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTC  >  W3110S.gb/1358410‑1358471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑