Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,428,979	C→T	T271I (ACC→ATC)	ynaA →	predicted tail protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,428,979	0	C	T	100.0%	13.7 / NA	6	T271I (ACC→ATC)	ynaA	predicted tail protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/6);  total (0/6)

GAGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAACCGGAATGTCGGCAAAACAAACGG  >  W3110S.gb/1428941‑1429002                                       |                        gAGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAATCGGAATGTCGGCAAAACAAACgg  <  1:1584732/62‑1 (MQ=255) gAGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAATCGGAATGTCGGCAAAACAAACgg  <  1:2075996/62‑1 (MQ=255) gAGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAATCGGAATGTCGGCAAAACAAACgg  <  1:2259549/62‑1 (MQ=255) gAGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAATCGGAATGTCGGCAAAACAAACgg  <  1:2350924/62‑1 (MQ=255)  aGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAATCGGAATGTCGGCAAAACAAACgg  <  1:1990112/61‑1 (MQ=255)   gTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAATCGGAATGTCGGCAAAACAAACgg  <  1:990624/60‑1 (MQ=255)                                       |                        GAGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAACCGGAATGTCGGCAAAACAAACGG  >  W3110S.gb/1428941‑1429002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑