Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,442,561 T→G intergenic (‑63/‑211) ydbK ← / → ydbJ fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,442,5610TG81.4% 187.6 / 24.6 70intergenic (‑63/‑211)ydbK/ydbJfused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/13);  new base G (0/57);  total (0/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

ATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATC  >  W3110S.gb/1442512‑1442616
                                                 |                                                       
aTTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa                                               <  1:1133160/60‑1 (MQ=255)
aTTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa                                               <  1:2735494/60‑1 (MQ=255)
aTTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa                                               <  1:2738876/60‑1 (MQ=255)
aTTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa                                               <  1:2656623/60‑1 (MQ=255)
aTTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa                                               <  1:2367014/60‑1 (MQ=255)
aTTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa                                               <  1:1910656/60‑1 (MQ=255)
aTTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa                                               <  1:396472/60‑1 (MQ=255)
aTTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa                                               <  1:2061847/60‑1 (MQ=255)
 ttGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa                                               <  1:1718469/59‑1 (MQ=255)
                           tgctgcGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCtt                  <  1:622063/62‑1 (MQ=255)
                           tgctgcGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCtt                  <  1:28166/62‑1 (MQ=255)
                           tgctgcGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCtt                  <  1:1529047/62‑1 (MQ=255)
                           tgctgcGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCtt                  <  1:1582185/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCTTTTTCGCTTTTGTGTCCCCCACATc  <  1:2345855/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2326377/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2344271/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2223466/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2349124/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2209676/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2208226/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2089824/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2188273/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2718759/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2801044/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2813643/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2855051/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2860849/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2893823/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:314310/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:485059/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:592879/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:655427/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:735610/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:859956/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:902157/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:938524/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1269253/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1362653/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:137999/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1438142/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1454883/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1458336/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1483690/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1496614/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1540752/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1559684/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1596080/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1263994/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1222783/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1756757/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1812033/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1920152/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1933573/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2018035/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2034538/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2040026/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2046043/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2086538/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2095854/62‑1 (MQ=255)
                                           gtaATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGCCCCCCACATc  <  1:2802178/62‑1 (MQ=255)
                                            taATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:984859/61‑1 (MQ=255)
                                            taATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1294326/61‑1 (MQ=255)
                                            taATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1339733/61‑1 (MQ=255)
                                            taATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1364806/61‑1 (MQ=255)
                                            taATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:584261/61‑1 (MQ=255)
                                            taATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1487079/61‑1 (MQ=255)
                                            taATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1657679/61‑1 (MQ=255)
                                            taATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:169748/61‑1 (MQ=255)
                                            taATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:2705872/61‑1 (MQ=255)
                                            taATCGTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATc  <  1:1031516/61‑1 (MQ=255)
                                                 |                                                       
ATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATC  >  W3110S.gb/1442512‑1442616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: