BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,487,157	T→C	L776L (TTG→CTG)	hrpA →	ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,487,157	0	T	C	86.4%	217.2 / 21.2	88	L776L (TTG→CTG)	hrpA	ATP‑dependent helicase
Reads supporting (aligned to +/- strand): ref base T (11/1); new base C (0/76); total (11/77)
Fisher's exact test for biased strand distribution p-value = 3.75e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

GTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCG  >  W3110S.gb/1487100‑1487206

gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt                                               <  1:1011356/62‑1 (MQ=255)

gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt                                               <  1:401868/62‑1 (MQ=255)

gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt                                               <  1:1344078/62‑1 (MQ=255)

gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt                                               <  1:724671/62‑1 (MQ=255)

gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt                                               <  1:193982/62‑1 (MQ=255)

gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt                                               <  1:2909577/62‑1 (MQ=255)

gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt                                               <  1:28376/62‑1 (MQ=255)

gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt                                               <  1:2763624/62‑1 (MQ=255)

gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt                                               <  1:1170582/62‑1 (MQ=255)

gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt                                               <  1:2521161/62‑1 (MQ=255)

gTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt                                               <  1:945291/62‑1 (MQ=255)

 tAGAAGAGCTGGAACACAAATCACGTCGCCGCTATATTCTGGTTGATGACGAAACgttgtt                                               <  1:1382613/61‑1 (MQ=255)

 tAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACgttgtt                                               <  1:2267340/61‑1 (MQ=255)

                             ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCAc                   >  1:1351480/1‑61 (MQ=255)

                             ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCAc                   >  1:163988/1‑61 (MQ=255)

                             ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  >  1:1980020/1‑62 (MQ=255)

                             ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  >  1:2305173/1‑62 (MQ=255)

                             ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  >  1:1918795/1‑62 (MQ=255)

                             ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  >  1:388341/1‑62 (MQ=255)

                             ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  >  1:2450074/1‑62 (MQ=255)

                             ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  >  1:1689102/1‑62 (MQ=255)

                             ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  >  1:833022/1‑62 (MQ=255)

                             ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  >  1:1165711/1‑62 (MQ=255)

                             ccgcGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  >  1:852370/1‑62 (MQ=255)

                               gcgATATTCTGGTGGATGACGAAATGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2514769/60‑1 (MQ=255)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTTAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1478933/60‑1 (MQ=38)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2572/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:824551/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2881912/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:284388/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:840604/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2803382/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2770449/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2625282/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:806696/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2538113/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:873251/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2526385/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:89551/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1021024/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:401752/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:52738/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:485960/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:47054/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:54255/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:464119/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:432364/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:404970/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:652721/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:541765/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:67269/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:374056/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:334404/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:329785/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:787815/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:322112/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2920533/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1331408/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2495815/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1713246/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1698288/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:169026/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:155572/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1553470/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1498270/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1489133/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1479245/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1359086/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1740574/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1308458/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1280982/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1280761/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1240628/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1225707/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1210969/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:120027/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1125312/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1098756/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2031554/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2409001/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2338509/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2338063/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2234000/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2206833/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2204869/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2173755/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2130866/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1785751/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2006109/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1981405/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:196483/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1921213/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1902830/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1882845/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1802689/60‑1 (MQ=39)

                               gcgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1799279/60‑1 (MQ=39)

                                cgATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1370393/59‑1 (MQ=39)

                                 gATATTCTGGTGGATGACGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:2249097/58‑1 (MQ=39)

                                             gatgaCGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCg  <  1:754981/62‑1 (MQ=255)

                                              atgaCGAAACGCTGTTTGAGTTCTACGACCAGCGCATCAGCCACg                  <  1:1938177/45‑1 (MQ=255)

GTAGAAGAGCTGGAACACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGACCAGCGCATCAGCCACGATGTAATCTCCGCTCG  >  W3110S.gb/1487100‑1487206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`