| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 70,581 | C→T | V65V (GTC→GTT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,581 | 0 | C | T | 97.8% | 1042.8 / 4.8 | 315 | V65V (GTC→GTT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (7/0); new base T (157/151); total (164/151) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
Only 100 of 316 total aligned reads displayed.
CTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTC > W3110S.gb/70525‑70636 | ctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCg < 1:861088/62‑1 (MQ=255) cAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc > 1:109719/1‑62 (MQ=255) tctcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc < 1:1947741/59‑1 (MQ=255) tctcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc < 1:2390953/59‑1 (MQ=255) tctcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc < 1:274688/59‑1 (MQ=255) tctcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc < 1:138690/59‑1 (MQ=255) tctcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc < 1:380419/59‑1 (MQ=255) tCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAttt < 1:2279292/62‑1 (MQ=255) tCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAttt > 1:281276/1‑62 (MQ=255) tCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAttt < 1:1054538/62‑1 (MQ=255) tCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAttt > 1:2632039/1‑62 (MQ=255) tCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtt > 1:659941/1‑61 (MQ=255) tCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGATTGCCGACCGGGTGATAtt > 1:2651974/1‑61 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc < 1:643610/61‑1 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc < 1:2233231/61‑1 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc < 1:1176999/61‑1 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc < 1:2332627/61‑1 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc < 1:2737651/61‑1 (MQ=255) gTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc < 1:1631494/60‑1 (MQ=255) ggtggtGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGc < 1:514061/62‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTc > 1:1721228/1‑56 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:32402/1‑61 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:411355/1‑61 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:593773/1‑61 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:2160058/1‑61 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:2097630/1‑61 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:614707/1‑61 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:896002/1‑61 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:1023844/1‑61 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:1374184/1‑61 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:1434449/1‑61 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:2779078/62‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:1739150/62‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:2661614/62‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:1693107/62‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:1006423/62‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:428825/62‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:1496360/62‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:607140/62‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:709681/62‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:741842/62‑1 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCATga < 1:231626/61‑1 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga < 1:2718849/61‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:249093/62‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:1707621/62‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:959648/62‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:2611106/62‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:1887780/62‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:2518344/62‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:804633/62‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:1516907/62‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:2035408/62‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:2008640/62‑1 (MQ=255) aaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAt < 1:1242380/62‑1 (MQ=255) aaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAt < 1:1824570/62‑1 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGCCCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:1310692/1‑62 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:2582214/1‑52 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:1617027/1‑52 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:1393585/1‑52 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:395294/1‑52 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:1413608/1‑52 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:1598839/1‑52 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:1464529/1‑52 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:2217008/1‑52 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:835251/1‑52 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:492454/1‑52 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:1535818/1‑52 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:1806878/1‑62 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:1754818/1‑62 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:2440008/1‑62 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:2412627/1‑62 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:1909836/1‑62 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:530486/1‑62 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGCGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:1484319/1‑62 (MQ=255) aTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:947905/1‑62 (MQ=255) ggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatc > 1:2914693/1‑61 (MQ=255) ggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatc > 1:1298503/1‑61 (MQ=255) ggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatc > 1:773712/1‑61 (MQ=255) ggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatc > 1:1257044/1‑61 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTa > 1:1064550/1‑62 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTa > 1:2084778/1‑62 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTa > 1:2354330/1‑62 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTa > 1:2240651/1‑62 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtc > 1:1868414/1‑62 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtc > 1:1671725/1‑62 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtc > 1:2469619/1‑62 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtc > 1:1130892/1‑62 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtc > 1:1567239/1‑62 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgt > 1:2848220/1‑61 (MQ=255) ttGTTTGACGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:1352332/38‑1 (MQ=37) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:1266782/42‑1 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:2190262/42‑1 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:213287/42‑1 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:577064/42‑1 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:2536588/42‑1 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:256946/42‑1 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:2768052/42‑1 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:346695/42‑1 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:1686502/42‑1 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:979124/42‑1 (MQ=255) | CTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTC > W3110S.gb/70525‑70636 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |