Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,493,597	T→C	intergenic (+33/+39)	ydcA → / ← hokB	hypothetical protein/toxic polypeptide, small

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,493,597	0	T	C	100.0%	112.2 / NA	34	intergenic (+33/+39)	ydcA/hokB	hypothetical protein/toxic polypeptide, small
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/34);  total (0/34)

CTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAA  >  W3110S.gb/1493557‑1493618                                         |                      cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:1266731/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:783538/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:778558/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:632899/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:56403/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:502003/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:465765/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2834843/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2829360/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2783532/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2638970/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2543107/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2519963/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2447447/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2359241/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2177781/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2158659/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:1270055/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:129602/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:193636/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:1809191/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:1654998/62‑1 (MQ=255) cTAACTGAAGTGTAAAAGGGGAGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2001674/62‑1 (MQ=255)  tAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:221858/61‑1 (MQ=255)  tAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:1243060/61‑1 (MQ=255)  tAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:1401641/61‑1 (MQ=255)  tAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:555219/61‑1 (MQ=255)  tAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:1751041/61‑1 (MQ=255)  tAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:1795257/61‑1 (MQ=255)  tAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2622322/61‑1 (MQ=255)  tAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2332729/61‑1 (MQ=255)  tAACTGAAGTGTAAAAGGGGGGCCATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:2643342/61‑1 (MQ=255)                        ccATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:875498/39‑1 (MQ=38)                         cATGAGAAAATGAATCCGCTAGTCAGATGCGCGGGGaa  <  1:1992289/38‑1 (MQ=38)                                         |                      CTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAA  >  W3110S.gb/1493557‑1493618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑