| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,541,247 | C→A | V1020F (GTC→TTC) | narZ ← | nitrate reductase 2 (NRZ), alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,541,247 | 0 | C | A | 76.6% | 72.1 / 30.5 | 47 | V1020F (GTC→TTC) | narZ | nitrate reductase 2 (NRZ), alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (6/5); new base A (0/36); total (6/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.30e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 | |||||||||||
CGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACG > W3110S.gb/1541198‑1541295 | ctgcACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGCCGTGATCGCTTTc < 1:1695081/59‑1 (MQ=255)ctgcACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTc < 1:1577931/59‑1 (MQ=255)ctgcACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTc < 1:1693266/59‑1 (MQ=255)ctgcACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTc < 1:404916/59‑1 (MQ=255)ctgcACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTc < 1:1971746/59‑1 (MQ=255) aTTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTccagaccaga < 1:1349932/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTccagaccagag < 1:1821497/62‑2 (MQ=38) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:548553/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:2675323/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:2710347/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:2783488/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:2916075/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:327949/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:37602/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:399793/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:428276/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:500354/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:2393338/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:584430/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:617878/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:619837/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:642291/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:708030/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:743950/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:858451/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:941071/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:1971397/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:1140920/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:1206225/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:1419763/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:1679953/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:1810098/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:207337/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:2278437/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:2287334/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:2358011/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:1005977/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:2515135/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:2547508/62‑1 (MQ=255) ttAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCTAGACCAGAc < 1:1732754/62‑1 (MQ=38) aaCTCATGAACGTTGGTGTATCCCGCGTTATAGGAGAAGTGATCGCTTTCcagaccagac < 1:1443191/60‑1 (MQ=37) gcgTTATAGGAGCCGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTAcg > 1:2013392/1‑62 (MQ=255) gcgTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTAcg > 1:354328/1‑62 (MQ=255) gcgTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTAcg > 1:2614576/1‑62 (MQ=255) gcgTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTAcg > 1:1706222/1‑62 (MQ=255) gcgTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTAcg > 1:159226/1‑62 (MQ=255) gcgTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTAcg > 1:1143842/1‑62 (MQ=255) | CGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACG > W3110S.gb/1541198‑1541295 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |