Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,542,394 C→T K637K (AAG→AAA)  narZ ← nitrate reductase 2 (NRZ), alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,542,3940CT77.9% 142.0 / 36.2 77K637K (AAG→AAA) narZnitrate reductase 2 (NRZ), alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (17/0);  new base T (60/0);  total (77/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGT  >  W3110S.gb/1542333‑1542421
                                                             |                           
cAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGtac                             <  1:1394905/62‑1 (MQ=255)
cAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGtac                             <  1:1081380/62‑1 (MQ=255)
cAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGtac                             <  1:1353691/62‑1 (MQ=255)
cAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGtac                             <  1:1115750/62‑1 (MQ=255)
cAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGtac                             <  1:532196/62‑1 (MQ=255)
cAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGtac                             <  1:1799545/62‑1 (MQ=255)
cAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGtac                             <  1:1283124/62‑1 (MQ=255)
 aGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGtac                             <  1:2899781/61‑1 (MQ=255)
 aGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGtac                             <  1:799721/61‑1 (MQ=255)
 aGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGtac                             <  1:1251584/61‑1 (MQ=255)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCACTCAGGTGACCGCTGTATTTACTg                       >  1:1593261/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGTAGACCGCTGtattta                          >  1:1416265/3‑59 (MQ=37)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGtatttac                         >  1:2704833/3‑60 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2864612/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2875634/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2889371/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2732089/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2890902/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2484808/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2515531/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:498833/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2478311/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2449475/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2422846/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2304715/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2298362/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2296003/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2254918/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:223905/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:711075/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:965750/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:945718/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:848012/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:824513/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:821523/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:812762/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:717909/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:716338/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2905689/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:652696/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:556534/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:532697/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:214767/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:417500/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:366885/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2911622/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1723381/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1071513/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1134816/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1223204/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1266328/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1302799/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1316408/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1549406/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1565701/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1566016/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1646095/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1659888/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1665954/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1729454/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1732734/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1809493/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2077075/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1016935/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1942014/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:183105/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1989933/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2071423/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2015554/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2098150/3‑62 (MQ=38)
      ggagccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:2068932/3‑62 (MQ=38)
      ggagcaagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGGTGACCGCTGTATTTACTg                       >  1:1539/3‑62 (MQ=255)
                          gcgcGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGaaa    >  1:2103947/1‑61 (MQ=255)
                          gcgcGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGaaa    >  1:1312313/1‑61 (MQ=255)
                          gcgcGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGaaa    >  1:56348/1‑61 (MQ=255)
                          gcgcGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGaaa    >  1:1247014/1‑61 (MQ=255)
                          gcgcGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGaaa    >  1:1201873/1‑61 (MQ=255)
                          gcgcGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGaaa    >  1:918211/1‑61 (MQ=255)
                          gcgcGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGaaa    >  1:22511/1‑61 (MQ=255)
                          gcgcGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGaaa    >  1:587499/1‑61 (MQ=255)
                          gcgcGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGaaa    >  1:2055503/1‑61 (MQ=255)
                          gcgcGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGaaa    >  1:1997932/1‑61 (MQ=255)
                          gcgcGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGaaa    >  1:366107/1‑61 (MQ=255)
                          gcgcGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGaaa    >  1:1754554/1‑61 (MQ=255)
                           cgcgAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGt  >  1:2287355/1‑62 (MQ=255)
                           cgcgAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGt  >  1:2197184/1‑62 (MQ=255)
                           cgcgAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGt  >  1:2791842/1‑62 (MQ=255)
                           cgcgAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGt  >  1:1091016/1‑62 (MQ=255)
                           cgcgAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGt  >  1:1778256/1‑62 (MQ=255)
                                                             |                           
CAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGT  >  W3110S.gb/1542333‑1542421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑