Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,549,933	G→A	A273A (GCG→GCA)	fdnG →	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,549,933	0	G	A	98.2%	174.3 / ‑4.4	54	A273A (GCG→GCA)	fdnG	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (0/53);  total (0/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCG  >  W3110S.gb/1549882‑1549946                                                    |              cGTTGTCGATCCCCGTTTTACGCGTCCCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1598222/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:292270/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1001388/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2286021/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2302808/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2367729/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2385452/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2441607/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2465454/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2533284/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2595112/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:261598/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:263458/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2648421/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2775760/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2197122/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:392867/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:448652/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:521539/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:555972/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:617965/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:725545/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:78259/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:843714/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:940772/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:941821/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:992243/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2153957/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1020196/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1098133/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1153825/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1165567/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1379524/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1404875/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1464720/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:151357/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1577848/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1596719/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1641482/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1647123/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1720053/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1842667/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1929615/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1945673/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2029043/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2030327/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:206481/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2069655/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2070853/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2127621/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2158098/61‑1 (MQ=255) cGTTGTCGATCCCCGTTTTACGCGTACCGCCTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:2885086/61‑1 (MQ=255)    tGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCg  <  1:2911886/62‑1 (MQ=255)                 tttACGCGTACCGCTTCTGTGGCGGATATTTACGCACCTATTCGt      <  1:1682833/45‑1 (MQ=255)                                                    |              CGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCG  >  W3110S.gb/1549882‑1549946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑