Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,590,693	A→G	L341L (CTT→CTC)	ydeT ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,590,693	0	A	G	100.0%	103.3 / NA	31	L341L (CTT→CTC)	ydeT	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/25);  total (6/25)

AGTTTTTATCTTTGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTAT  >  W3110S.gb/1590647‑1590745                                               |                                                     aGTTTTTATCTTTGCCCCATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATaa                                       >  1:2244055/1‑62 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:2547950/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:720986/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:692513/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:65325/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:633922/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:54091/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:479414/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:407072/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:406613/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:343390/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:312605/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:2907529/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:2764420/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:2709808/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:2690682/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:2559163/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:2458575/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:245841/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:2261560/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:2128724/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:2054463/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:1599410/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:1420262/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:1392812/61‑1 (MQ=255)               ccccATGAAACCTGTAATTGCCCTGACTGTGGGAGTCCAGTCAGATAAACCTGACCAtttt                          <  1:1210925/61‑1 (MQ=255)                                      tGACTGTGGGAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTAt  >  1:1015169/1‑62 (MQ=255)                                      tGACTGTGGGAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTAt  >  1:2523416/1‑62 (MQ=255)                                      tGACTGTGGGAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTAt  >  1:2077582/1‑62 (MQ=255)                                      tGACTGTGGGAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTAt  >  1:1559663/1‑62 (MQ=255)                                      tGACTGTGGGAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTAt  >  1:1428269/1‑62 (MQ=255)                                               |                                                     AGTTTTTATCTTTGCCCCATGAAACCTGTAATTGCCCTGACTGTGGAAGTCCAGTCAGATAAACCTGACCATTTTCCGCGACAATGCTGCCATTTTTAT  >  W3110S.gb/1590647‑1590745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑