Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	70,740	T→G	G118G (GGT→GGG)	araC →	DNA‑binding transcriptional dual regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	70,740	0	T	G	100.0%	253.7 / NA	74	G118G (GGT→GGG)	araC	DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (54/20);  total (54/20)

CGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAA  >  W3110S.gb/70687‑70794                                                      |                                                       cgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGTGGTTCTTTCg                                                >  1:2543958/1‑62 (MQ=255) cgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctttag                                                >  1:2386036/1‑60 (MQ=255) cgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCg                                                >  1:2083560/1‑62 (MQ=255) cgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCg                                                >  1:847638/1‑62 (MQ=255) cgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCg                                                >  1:2207687/1‑62 (MQ=255) cgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCg                                                >  1:117894/1‑62 (MQ=255) cgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCg                                                >  1:1838621/1‑62 (MQ=255) cgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCg                                                >  1:2271544/1‑62 (MQ=255) cgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCg                                                >  1:391941/1‑62 (MQ=255) cgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCg                                                >  1:2303601/1‑62 (MQ=255)       tACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa                                          <  1:924499/62‑1 (MQ=255)       tACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa                                          <  1:562036/62‑1 (MQ=255)       tACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa                                          <  1:305148/62‑1 (MQ=255)       tACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa                                          <  1:2443647/62‑1 (MQ=255)       tACTGGCATGAATGGCTTAACTGGCCGTCAATAATTGCCAATACGGGGTTCTTTCGCCCGGa                                          <  1:2539627/62‑1 (MQ=255)        aCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa                                          <  1:1096054/61‑1 (MQ=255)        aCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa                                          <  1:74246/61‑1 (MQ=255)                        tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGccac                         >  1:1844289/1‑60 (MQ=255)                        tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc                         >  1:2788322/1‑62 (MQ=255)                        tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc                         >  1:2873481/1‑62 (MQ=255)                        tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc                         >  1:2275621/1‑62 (MQ=255)                        tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc                         >  1:442825/1‑62 (MQ=255)                        tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc                         >  1:80335/1‑62 (MQ=255)                        tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc                         >  1:976031/1‑62 (MQ=255)                        tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc                         >  1:2064081/1‑62 (MQ=255)                        tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc                         >  1:2025465/1‑62 (MQ=255)                        tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc                         >  1:1255654/1‑62 (MQ=255)                        tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc                         >  1:1295771/1‑62 (MQ=255)                        tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc                         >  1:1528531/1‑62 (MQ=255)                                   cAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              <  1:2849227/62‑1 (MQ=255)                                   cAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              <  1:1108040/62‑1 (MQ=255)                                   cAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              <  1:335964/62‑1 (MQ=255)                                   cAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              <  1:1353472/62‑1 (MQ=255)                                   cAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              <  1:2902666/62‑1 (MQ=255)                                   cAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              <  1:2892278/62‑1 (MQ=255)                                   cAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              <  1:1718600/62‑1 (MQ=255)                                   cAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              <  1:1753787/62‑1 (MQ=255)                                   cAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              <  1:2195468/62‑1 (MQ=255)                                   cAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              <  1:1840938/62‑1 (MQ=255)                                    aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              <  1:2663293/61‑1 (MQ=255)                                    aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              <  1:455273/61‑1 (MQ=255)                                          tGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              <  1:1612130/55‑1 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa                   >  1:831842/1‑45 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc              >  1:323872/1‑50 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACGTGTTTGGGCaa  >  1:1460211/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:1288811/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:944130/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:473506/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:943814/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:1110472/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:525021/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:1998447/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:581879/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:2012621/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:123544/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:772526/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:1156091/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:1151802/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:362520/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:1792863/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:1695823/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:2398811/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:2440971/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:2510182/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:1601137/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:2246274/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:2551535/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:2003040/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa  >  1:188590/1‑62 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa   >  1:2638472/1‑61 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa   >  1:2742652/1‑61 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa   >  1:1404770/1‑61 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa   >  1:1956642/1‑61 (MQ=255)                                               aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa   >  1:2048257/1‑61 (MQ=255)                                               aTACGGGGTTCTTTAGCCCGGATGAAGCGCACCAGCCGCATTTCAGAGACCTGTTTGGGCaa  >  1:729894/1‑62 (MQ=255)                                                      |                                                       CGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAA  >  W3110S.gb/70687‑70794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑