Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,628,270	T→C	L275L (CTA→CTG)	dcp ←	dipeptidyl carboxypeptidase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,628,270	0	T	C	100.0%	237.2 / NA	69	L275L (CTA→CTG)	dcp	dipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (69/0);  total (69/0)

TTTTCCATGCGGCATAATGAGGAAAACCAAGTAGTGTTGCCTGTTGTGCACGGATCTCCAC  >  W3110S.gb/1628239‑1628299                                |                              ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTgcgcgc            >  1:2407988/1‑49 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGGGCGGATCTcc    >  1:1821117/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGAtctca    >  1:1899419/1‑58 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGAtctc     >  1:2071544/1‑58 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGAtctc     >  1:1595266/1‑58 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTccac  >  1:2233599/1‑61 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2411855/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2921605/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2917009/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2841830/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2706529/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2628417/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2476833/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2460957/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2922519/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2389145/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2249182/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2166142/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:216017/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:515605/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:987943/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:981892/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:965353/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:948708/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:798296/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:651821/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:57733/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:557888/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:209138/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:515503/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:45287/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:408605/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:351690/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:336615/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:324196/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:297521/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2925933/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1406959/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1021971/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1030042/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1046948/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1058868/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1075389/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1113626/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1125140/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1168947/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1180826/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1209259/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1220316/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1230077/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1307246/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1340704/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1346508/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2145980/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1506126/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1525176/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1552411/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1565949/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1630132/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1630903/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1710291/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1711267/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1871397/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1883974/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1950800/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1958644/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2049109/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:1009258/1‑59 (MQ=255) ttttCCATGCGGCATAATGAGGAAAACCAAGCAGTTTCGCCTGCTGCGCGCGGATCTcc    >  1:2109521/1‑59 (MQ=255)                                |                              TTTTCCATGCGGCATAATGAGGAAAACCAAGTAGTGTTGCCTGTTGTGCACGGATCTCCAC  >  W3110S.gb/1628239‑1628299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑