Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,665,680 T→G F120V (TTC→GTC)  ynfH → oxidoreductase, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,665,6800TG87.1% 261.2 / 16.5 85F120V (TTC→GTC) ynfHoxidoreductase, membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/11);  new base G (0/74);  total (0/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

ATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACC  >  W3110S.gb/1665647‑1665721
                                 |                                         
aTGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGt               <  1:1932880/62‑1 (MQ=255)
aTGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGt               <  1:655349/62‑1 (MQ=255)
aTGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGt               <  1:2689640/62‑1 (MQ=255)
aTGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGt               <  1:2218351/62‑1 (MQ=255)
aTGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGt               <  1:461648/62‑1 (MQ=255)
aTGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGt               <  1:1217440/62‑1 (MQ=255)
aTGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGt               <  1:800782/62‑1 (MQ=255)
aTGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGt               <  1:1125209/62‑1 (MQ=255)
aTGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGt               <  1:89206/62‑1 (MQ=255)
aTGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGt               <  1:2339047/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCTGCATCATTTTCGTCTGGATGATGAcc  <  1:472643/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTGGTCTGGATGATGAcc  <  1:611822/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGAtgatgagc  <  1:245144/62‑3 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2913832/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2845818/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:293544/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2825750/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2689030/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2759994/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2733839/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2403285/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2617366/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2532919/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2461582/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2425654/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2377490/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:718354/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:995833/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:893599/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:889827/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:880728/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:798772/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:748193/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:744709/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:720707/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:353499/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:701060/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:685561/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:525471/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:478516/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:431659/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:37780/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:367789/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:138029/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1767669/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1628338/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1018065/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1573301/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1555237/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1554131/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1553837/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1394193/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1577824/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1306608/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:127424/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1188767/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:11829/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1124383/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1090845/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1057322/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1025458/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2056613/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2227419/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2183724/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:225057/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2286221/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2175921/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2291019/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2060133/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:202764/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1982912/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2292781/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1949700/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:193689/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1893627/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1786842/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATAAcc  <  1:730670/62‑1 (MQ=255)
             tGGGGAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTCTCGTCTGGATGATGAcc  <  1:2409652/62‑1 (MQ=255)
             aggggAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:2109407/61‑1 (MQ=255)
              ggggAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGt               <  1:1035587/48‑1 (MQ=255)
              ggggAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1283453/61‑1 (MQ=255)
              ggggAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:854454/61‑1 (MQ=255)
              ggggAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:733964/61‑1 (MQ=255)
              ggggAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:582084/61‑1 (MQ=255)
              ggggAAACTCTGGTTACTTGTCAGCATGGCGCTCGGCATCATTTTCGTCTGGATGATGAcc  <  1:1700988/61‑1 (MQ=255)
                                 |                                         
ATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACC  >  W3110S.gb/1665647‑1665721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑