| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 71,079 | C→T | R231R (CGC→CGT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,079 | 0 | C | T | 99.7% | 1051.5 / ‑5.3 | 309 | R231R (CGC→CGT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (99/209); total (100/209) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
Only 100 of 309 total aligned reads displayed.
CCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGT > W3110S.gb/71034‑71132 | ccAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAg > 1:1514189/1‑61 (MQ=255) cagcatTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGTCGAAgct < 1:2840517/62‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct < 1:1031790/62‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct < 1:1776960/62‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct < 1:2031200/62‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct < 1:2086759/62‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct < 1:1581974/62‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct < 1:1394201/62‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct < 1:2550149/62‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct < 1:480279/62‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct < 1:788450/62‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct < 1:764130/62‑1 (MQ=255) cagcagTTAGGGATTAGCGGCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct < 1:1422699/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:2566445/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:193834/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:1944832/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:1991433/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:91674/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:1645895/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:162451/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:83441/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:332816/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:2307410/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:1246921/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:1482135/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:2395815/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:619055/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:1336654/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:2399546/62‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:593207/62‑1 (MQ=255) gcacTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:1813367/62‑1 (MQ=255) agTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCtt < 1:216739/62‑1 (MQ=255) agTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCtt < 1:2328435/62‑1 (MQ=255) agTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCtt < 1:2814904/62‑1 (MQ=255) agTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCtt < 1:1064393/62‑1 (MQ=255) gTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCtt < 1:942239/61‑1 (MQ=255) gTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCtt < 1:515003/61‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTTCTTTTGAg < 1:2651408/61‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:260403/62‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:1280810/62‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:2519312/62‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:2676821/62‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:2750270/62‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:460429/62‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:1135469/62‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:722145/62‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:784988/62‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:793727/62‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:2183423/62‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:2921845/62‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:1971281/62‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCACGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:569448/62‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:2235786/52‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:1221333/52‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:1122893/52‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:1556666/52‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:2856312/52‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:2863404/52‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:171569/52‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:1751209/52‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:37290/52‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:1676862/61‑1 (MQ=255) tAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCAcca > 1:2410781/1‑60 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGcaccac > 1:1178420/1‑62 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGcaccac > 1:586983/1‑62 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGcaccac > 1:10936/1‑62 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGcaccac > 1:2046613/1‑62 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCAcca > 1:1147696/1‑59 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCAcca > 1:437148/1‑59 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCAcca > 1:411742/1‑59 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCtt < 1:2123013/50‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:1838420/45‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg > 1:11600/1‑52 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg > 1:545746/1‑52 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg > 1:886667/1‑52 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg > 1:2801707/1‑52 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg > 1:1910046/1‑52 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg > 1:1929654/1‑52 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg > 1:1591660/1‑52 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg > 1:1889860/1‑52 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg > 1:1011362/1‑52 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGa > 1:1078737/1‑36 (MQ=25) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa > 1:2478908/1‑51 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg > 1:2382000/1‑62 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg > 1:863677/1‑62 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg > 1:2256187/1‑62 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg > 1:1523370/1‑62 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg > 1:2334326/1‑62 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg > 1:2365776/1‑62 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg > 1:66320/1‑62 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg > 1:363060/1‑62 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg > 1:1313508/1‑62 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg > 1:2586791/1‑62 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg > 1:1261501/1‑62 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg > 1:305700/1‑62 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg > 1:327191/1‑62 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGAACCACCCGGATg > 1:14729/1‑62 (MQ=255) gACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgt < 1:243262/62‑1 (MQ=255) gACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgt < 1:486970/62‑1 (MQ=255) aCCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgt < 1:1322948/61‑1 (MQ=255) | CCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGT > W3110S.gb/71034‑71132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |